Longevity of B-cell and T-cell responses after live attenuated influenza vaccination in children.
Longevity of B-cell and T-cell responses after live attenuated influenza vaccination in children.
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DOI：
10.1093/infdis/jiu654
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发表时间：
2015-05-15
期刊：
影响因子：
--
通讯作者：
Cox RJ
中科院分区：
文献类型：
--
作者：
Mohn KG;Bredholt G;Brokstad KA;Pathirana RD;Aarstad HJ;Tøndel C;Cox RJ
背景：流感减毒活疫苗（LAIV）是儿童的首选疫苗，但保护性免疫应答背后的机制尚不清楚，免疫持续时间仍有待阐明。 本研究报告了LAIV引起的B细胞和T细胞应答的寿命。方法：38名儿童（3-17岁）接受季节性LAIV。 在疫苗接种前采集血样，并在疫苗接种后1年内连续采样。通过血凝抑制试验评价体液应答，通过酶联免疫吸附斑点试验（ELISpot）评价记忆B细胞应答。通过干扰素γ（IFN-γ）ELISpot分析评价T细胞应答，并使用流式细胞术进行CD 4 + T细胞的细胞内细胞因子染色以检测IFN-γ、白细胞介素2和肿瘤坏死因子α。结果：LAIV诱导B细胞和T细胞应答显著增加，并在接种后至少持续1年。 观察到菌株变异，其中B菌株引起更强的反应。IFN-γ表达的T细胞计数显著增加，1年后仍高于接种前水平。1次给药后，1型辅助性T细胞内细胞因子（白细胞介素2、IFN-γ和肿瘤坏死因子α）的表达增加，第2次给药后增强。血凝抑制滴度持续1年。疫苗诱导的记忆B细胞计数显著增加，并且应答持续一年。结论：LAIV引起的B细胞和T细胞反应在儿童中持续至少1年。 这是一个新的发现，将有助于未来的疫苗政策。
Background. The live attenuated influenza vaccine (LAIV) is the preferred vaccine for children, but the mechanisms behind protective immune responses are unclear, and the duration of immunity remains to be elucidated. This study reports on the longevity of B-cell and T-cell responses elicited by the LAIV. Methods. Thirty-eight children (3–17 years old) were administered seasonal LAIV. Blood samples were collected before vaccination with sequential sampling up to 1 year after vaccination. Humoral responses were evaluated by a hemagglutination inhibition assay, and memory B-cell responses were evaluated by an enzyme-linked immunosorbent spot assay (ELISpot). T-cell responses were evaluated by interferon γ (IFN-γ) ELISpot analysis, and intracellular cytokine staining of CD4+ T cells for detection of IFN-γ, interleukin 2, and tumor necrosis factor α was performed using flow cytometry. Results. LAIV induced significant increases in B-cell and T-cell responses, which were sustained at least 1 year after vaccination. Strain variations were observed, in which the B strain elicited stronger responses. IFN-γ–expressing T cell counts increased significantly, and remained higher than prevaccination levels 1 year later. Expression of T-helper type 1 intracellular cytokines (interleukin 2, IFN-γ, and tumor necrosis factor α) increased after 1 dose and were boosted after the second dose. Hemagglutination inhibition titers were sustained for 1 year. Vaccine-induced memory B cell counts were significantly increased, and the response persisted for one year. Conclusions. LAIV elicited B-cell and T-cell responses that persisted for at least 1 year in children. This is a novel finding that will aid future vaccine policy.
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影响因子：
3.2
作者：
McKinstry, K. Kai;Dutton, Richard W.;Swain, Susan L.;Strutt, Tara M.
通讯作者：
Strutt, Tara M.
影响因子：
5.5
作者：
Cherukuri, Anu;Servat, Esteban;Woo, Jennifer
通讯作者：
Woo, Jennifer
影响因子：
5.1
作者：
Belshe, RB;Gruber, WC;Wolff, M
通讯作者：
Wolff, M
影响因子：
3.6
作者：
Lee, MS;Mahmood, K;Mendelman, PM
通讯作者：
Mendelman, PM
影响因子：
158.5
作者：
Belshe, Robert B.;Edwards, Kathryn M.;Connor, Edward M.
通讯作者：
Connor, Edward M.