Effects of Rehabilitation on Perineural Nets and Synaptic Plasticity Following Spinal Cord Transection.
Effects of Rehabilitation on Perineural Nets and Synaptic Plasticity Following Spinal Cord Transection.
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康复训练对脊髓横断后神经周围神经网络和突触可塑性的影响
DOI：
10.3390/brainsci10110824
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发表时间：
2020-11-06
期刊：
影响因子：
3.3
通讯作者：
Ichiyama RM
中科院分区：
文献类型：
--
作者：
Al'joboori YD;Edgerton VR;Ichiyama RM
腰部脊髓硬膜外电刺激 (ES) 与日常运动训练相结合已被证明可以增强啮齿类动物完全脊柱横断和人类临床完全脊柱损伤后的迈步能力。尽管观察到功能增益，但与这种恢复相关的可塑性机制仍不清楚。在这里，我们研究了 ES 和运动训练如何影响硫酸软骨素蛋白聚糖 (CSPG)、神经元周围网 (PNN) 的表达和脊髓运动神经元的突触可塑性。为了测试这一点，成年大鼠接受了完整的脊柱横断（T9-T10），然后从受伤后 7 天（dpi）开始在 ES 下进行日常运动训练，并给予奎帕嗪（一种血清素 (5-HT) 激动剂）。除了 PNN 和 CSPG 表达之外，还在 7、21 和 67 dpi 时检查兴奋性和抑制性突触变化。损伤后CSPG表达总量随时间显着增加，训练无影响。一个有趣的发现是 γ-运动神经元不表达 PNN，而 α-运动神经元则表现出明确定义的 PNN。这种显着差异反映在 γ 运动神经元中观察到的突触变化程度比 α 运动神经元更大。发现 CSPG 表达与 α 运动神经元周围推定突触的变化之间存在中等负相关，但与 γ 运动神经元没有相关性。这些结果表明，γ-运动神经元活性的调节是与完全脊柱横断后 ES 下运动训练诱导的功能恢复相关的重要机制。
Epidural electrical stimulation (ES) of the lumbar spinal cord combined with daily locomotor training has been demonstrated to enhance stepping ability after complete spinal transection in rodents and clinically complete spinal injuries in humans. Although functional gain is observed, plasticity mechanisms associated with such recovery remain mostly unclear. Here, we investigated how ES and locomotor training affected expression of chondroitin sulfate proteoglycans (CSPG), perineuronal nets (PNN), and synaptic plasticity on spinal motoneurons. To test this, adult rats received a complete spinal transection (T9–T10) followed by daily locomotor training performed under ES with administration of quipazine (a serotonin (5-HT) agonist) starting 7 days post-injury (dpi). Excitatory and inhibitory synaptic changes were examined at 7, 21, and 67 dpi in addition to PNN and CSPG expression. The total amount of CSPG expression significantly increased with time after injury, with no effect of training. An interesting finding was that γ-motoneurons did not express PNNs, whereas α-motoneurons demonstrated well-defined PNNs. This remarkable difference is reflected in the greater extent of synaptic changes observed in γ-motoneurons compared to α-motoneurons. A medium negative correlation between CSPG expression and changes in putative synapses around α-motoneurons was found, but no correlation was identified for γ-motoneurons. These results suggest that modulation of γ-motoneuron activity is an important mechanism associated with functional recovery induced by locomotor training under ES after a complete spinal transection.
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影响因子：
14.5
作者：
Maier, Irin C.;Ichiyama, Ronaldo M.;Schwab, Martin E.
通讯作者：
Schwab, Martin E.
影响因子：
5.3
作者：
ChenKinon;Marsh, Barnaby C.;Ichiyama, Ronaldo M.
通讯作者：
Ichiyama, Ronaldo M.
影响因子：
2.5
作者：
Ichiyama, Ronaldo M.;Brown, Jonas;Havton, Leif A.
通讯作者：
Havton, Leif A.
DOI：
10.1007/bf00315460
发表时间：
1986-01-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
GRIBNAU, AAM;DEKORT, EJM;NIEUWENHUYS, R
通讯作者：
NIEUWENHUYS, R
影响因子：
5.3
作者：
Lemons, ML;Howland, DR;Anderson, DK
通讯作者：
Anderson, DK