Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.
Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.
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Fractalkine 在人卵巢中表达，并增加人黄素化颗粒细胞中的孕酮生物合成
DOI：
10.1186/1477-7827-9-95
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发表时间：
2011-06-30
期刊：
影响因子：
--
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Huang S;Zhao P;Yang L;Chen Y;Yan J;Duan E;Qiao J
最近来自啮齿类动物卵巢的证据表明fractalkine的表达和fractalkine受体的存在，并表明fractalkine在人卵巢中的作用显著增加，但fractalkine和CX3CR1在人卵巢中的作用尚不清楚。本研究旨在测定fractalkine和CX3CR1在人卵巢中的表达水平，并探讨它们在促黄体生成素颗粒细胞合成性激素中的作用。这是首次详细报道fractalkine和CX3CR1在人类卵巢中的表达和功能。采用免疫组化方法检测5例卵巢病理标本中Fractalkine和CX3CR1的表达水平。颗粒细胞是在体外受精治疗期间从患者身上获得的。他们被培养和用增加剂量的hCG治疗，有或没有fractalkine。收集培养液，化学发光法检测雌二醇和黄体酮。real-time RT-PCR检测加或不加fractalkine处理的颗粒细胞中StAR、3-βHSD和CYP11A的表达。Fractalkine和CX3CR1在人卵巢和黄体生成素颗粒细胞中表达。然而，fractalkine在黄体生成素颗粒细胞中的表达更强。fractalkine增加了hCG刺激孕酮产生的剂量依赖性，同时增加了关键甾体生成酶（StAR， 3-βHSD和CYP11A）的转录水平，但对雌二醇的生物合成没有影响（P < 0.05）。Fractalkine和CX3CR1在人卵巢和黄体生成素颗粒细胞中表达。Fractalkine可以通过提高关键类固醇生成酶的转录水平，以剂量依赖性的方式增加黄体酮的生物合成。
Recent evidence from rodent ovaries has demonstrated expression of fractalkine and the existence of fractalkine receptor, and showed that there is a significant increase in steroidogenesis in response to fractalkine, yet the role of fractalkine and CX3CR1 in the human ovary is still unknown. This study aimed to determine the expression levels of fractalkine and CX3CR1 in the human ovary and to investigate their roles in sexual hormone biosynthesis by human luteinising granulosa cells. This is the first detailed report of fractalkine and CX3CR1 expression and function in the human ovary. Fractalkine and CX3CR1 expression levels were measured by immunohistochemistry using ovarian tissue from pathological specimens from five individuals. Granulosa cells were obtained from patients during IVF treatment. They were cultured and treated with increasing doses of hCG with or without fractalkine. Media were collected to detect estradiol and progesterone by chemiluminescence. StAR, 3-βHSD and CYP11A expression were determined in granulosa cells treated with or without fractalkine by real-time RT-PCR. Fractalkine and CX3CR1 were expressed in the human ovary and in luteinising granulosa cells. However, fractalkine expression was stronger in luteinising granulosa cells. Treatment with fractalkine augmented hCG stimulation of progesterone production in a dose-dependent manner with concomitant increases in transcript levels for key steroidogenic enzymes (StAR, 3-βHSD and CYP11A) but had no effect on estradiol biosynthesis(P < 0.05). Fractalkine and CX3CR1 were found to express in human ovary and luteinising granulosa cells. Fractalkine can increase the biosynthesis of progesterone in a dose-dependent manner by enhancing transcript levels of key steroidogenic enzymes.
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DOI：
10.1073/pnas.95.18.10896
发表时间：
1998-09-01
影响因子：
11.1
作者：
Harrison, JK;Jiang, Y;Feng, LL
通讯作者：
Feng, LL
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR
影响因子：
--
作者：
Ben-Shlomo, Izhar;Rauch, Rami;Hsueh, Aaron J. W.
通讯作者：
Hsueh, Aaron J. W.
影响因子：
4.8
作者：
Gasic, S;Bodenburg, Y;Urban, RJ
通讯作者：
Urban, RJ
DOI：
10.1186/1477-7827-1-90
发表时间：
2003-11-10
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Christenson, Lane K;Devoto, Luigi
通讯作者：
Devoto, Luigi