Protective unfolded protein response in human pancreatic beta cells transplanted into mice.
Protective unfolded protein response in human pancreatic beta cells transplanted into mice.
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DOI：
10.1371/journal.pone.0011211
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发表时间：
2010-06-18
期刊：
影响因子：
3.7
通讯作者：
Weir GC
中科院分区：
文献类型：
--
作者：
Kennedy J;Katsuta H;Jung MH;Marselli L;Goldfine AB;Balis UJ;Sgroi D;Bonner-Weir S;Weir GC
There is great interest about the possible contribution of ER stress to the apoptosis of pancreatic beta cells in the diabetic state and with islet transplantation. Expression of genes involved in ER stress were examined in beta cell enriched tissue obtained with laser capture microdissection (LCM) from frozen sections of pancreases obtained from non-diabetic subjects at surgery and from human islets transplanted into ICR-SCID mice for 4 wk. Because mice have higher glucose levels than humans, the transplanted beta cells were exposed to mild hyperglycemia and the abnormal environment of the transplant site. RNA was extracted from the LCM specimens, amplified and then subjected to microarray analysis. The transplanted beta cells showed an unfolded protein response (UPR). There was activation of many genes of the IRE-1 pathway that provide protection against the deleterious effects of ER stress, increased expression of ER chaperones and ERAD (ER-associated protein degradation) proteins. The other two arms of ER stress, PERK and ATF-6, had many down regulated genes. Downregulation of EIF2A could protect by inhibiting protein synthesis. Two genes known to contribute to apoptosis, CHOP and JNK, were downregulated. Human beta cells in a transplant site had UPR changes in gene expression that protect against the proapoptotic effects of unfolded proteins.
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影响因子：
5.8
作者：
Carlsson, PO;Palm, F;Mattsson, G
通讯作者：
Mattsson, G
影响因子：
7.7
作者：
Huang, Chang-jiang;Lin, Chia-yu;Butler, Peter C.
通讯作者：
Butler, Peter C.
DOI：
10.1083/jcb.200904060
发表时间：
2009-09-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Li G;Mongillo M;Chin KT;Harding H;Ron D;Marks AR;Tabas I
通讯作者：
Tabas I
影响因子：
8.2
作者：
Cantley, J.;Choudhury, A. I.;Withers, D. J.
通讯作者：
Withers, D. J.
影响因子：
3.2
作者：
Araki, E;Oyadomari, S;Mori, M
通讯作者：
Mori, M