Comparison of 4 direct Coombs' test methods with polyclonal antiglobulins in anemic and nonanemic dogs for in-clinic or laboratory use.
Comparison of 4 direct Coombs' test methods with polyclonal antiglobulins in anemic and nonanemic dogs for in-clinic or laboratory use.
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DOI：
10.1111/jvim.12292
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发表时间：
2014-03
影响因子：
2.6
通讯作者：
Giger U
中科院分区：
文献类型：
--
作者：
Caviezel LL;Raj K;Giger U
直接抗球蛋白试验（DAT）的困难及其对犬免疫介导的溶血性贫血（IMHA）明显缺乏灵敏度和特异性，引起了对其诊断价值的怀疑。比较犬的不同DAT和其他血液学参数。来自研究群体和兽医诊所的59只非贫血犬和46只贫血犬（± IMHA）的抗凝血液样本。前瞻性观察性研究：免疫层析条，凝胶微柱，和毛细管技术进行了比较与标准微量DAT使用2多价抗球蛋白。评估了球形红细胞增多症、自身凝集、渗透脆性和临床数据。所有59只非贫血犬的血样均为DAT-。在46只贫血犬中，33只疑似IMHA，但只有20只DAT+。新旧DAT方法即使在冷冻血样储存1周后也能获得相当且一致的结果。分别在15只和16只DAT+犬中观察到球形红细胞增多症和自身凝集（清洗后未持续）。其他26只贫血犬（包括21只既往输血犬和4只自身凝集犬）通过其他方法检测DAT。70%（19/27）的贫血犬和所有15只接受测试的DAT+犬的渗透脆性均增加。有限的随访测试显示DAT+结果持续3 - 70天。新的条带和毛细管DAT方法是有前途的辅助临床工具。尽管既往接受过免疫抑制治疗并存在自身凝集，但在患有IMHA的贫血犬中DAT呈阳性。输血不会导致错误的DAT+结果。我们的研究结果支持DAT作为诊断犬IMHA的基石。
Difficulties with the direct antiglobulin test (DAT) and its apparent lack of sensitivity and specificity for immune‐mediated hemolytic anemia (IMHA) in dogs have raised skepticism regarding its diagnostic value. To compare different DATs and other hematologic parameters in dogs. Anticoagulated blood samples from 59 nonanemic and 46 anemic dogs (± IMHA) from a research colony and veterinary clinics. Prospective observational study: Immunochromatographic strip, gel microcolumn, and capillary techniques were compared with standard microtiter DAT using 2 polyvalent antiglobulins. Spherocytosis, autoagglutination, osmotic fragility, and clinical data were assessed. Blood samples from all 59 nonanemic dogs were DAT‐. Among 46 anemic dogs, 33 were suspected of IMHA, but only 20 were DAT+. Old and new DAT methods yielded comparable and consistent results even after storage of chilled blood samples for 1 week. Spherocytosis and autoagglutination (that did not persist after washing) were noted in 15 and 16 DAT+ dogs, respectively. The other 26 anemic dogs, including 21 previously transfused dogs and 4 with autoagglutination, tested DAT‐ by the other methods. Osmotic fragility was increased in 70% (19/27) of anemic and all 15 DAT+ dogs tested. Limited follow‐up testing revealed DAT+ results for 3–70 days. The novel strip and capillary DAT methods are promising adjunct in‐clinic tools. Despite prior immunosuppressive treatment and presence of autoagglutination, the DAT was positive in anemic dogs with IMHA. Transfusion did not cause false DAT+ results. Our results support DAT as a cornerstone in the diagnosis of canine IMHA.
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影响因子：
1.8
作者：
SLAPPENDEL, RJ
通讯作者：
SLAPPENDEL, RJ
影响因子：
1.2
作者：
Lanaux, Travis M.;Rozanski, Elizabeth A.;Knoll, Joyce S.
通讯作者：
Knoll, Joyce S.
影响因子：
2.4
作者：
JONES, DRE;GRUFFYDDJONES, TJ;BOURNE, FJ
通讯作者：
BOURNE, FJ
影响因子：
1.2
作者：
Tan, Emmeline;Bienzle, Dorothee;Wood, Darren
通讯作者：
Wood, Darren
影响因子：
1.2
作者：
Overmann, Jed A.;Sharkey, Leslie C.;Borjesson, Dori L.
通讯作者：
Borjesson, Dori L.