A Gq-Ca2+ axis controls circuit-level encoding of circadian time in the suprachiasmatic nucleus.
A Gq-Ca2+ axis controls circuit-level encoding of circadian time in the suprachiasmatic nucleus.
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DOI：
10.1016/j.neuron.2013.03.011
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发表时间：
2013-05-22
期刊：
影响因子：
16.2
通讯作者：
Hastings MH
中科院分区：
文献类型：
--
作者：
Brancaccio M;Maywood ES;Chesham JE;Loudon AS;Hastings MH
细胞内转录/翻译后反馈环（TTFL）在视交叉上核（SCN）的昼夜节律起搏器中的作用已得到充分证实。相比之下，G-偶联途径和胞质节律对SCN起搏的细胞间控制的贡献知之甚少。因此，我们将SCN切片的病毒转导与荧光/生物发光成像相结合，以可视化GCaMP 3报告的细胞内钙[Ca 2 +]i的昼夜节律振荡以及Ca 2 +/cAMP响应元件的激活。我们将它们在时间和SCN空间上相位映射到TTFL，并证明它们依赖于G-偶联血管活性肠肽（VIP）信号。药物遗传学操作揭示了Gq、Gs和Gi对细胞溶质和TTFL昼夜节律的个体贡献。重要的是，在少数神经元中激活Gq依赖性（但不是Gs或Gi）通路重编程[Ca 2 +]i和TTFL节律跨越整个SCN。这种重编程是由内在的VIPergic信号转导介导的，从而揭示了Gq/[Ca 2 +]i-VIP主基序和SCN昼夜节律时间网络编码内的意外可塑性。SCN [Ca 2 +]i和TTFL昼夜节律景观相位图通过实时成像绘制少数神经元中Gq-[Ca 2 +]i轴的募集重新编程SCN网络通过内在VIPergic信号传导介导的选择性Gq介导的重新编程揭示了SCN昼夜节律网络的内部结构和意外的可塑性揭示了视交叉上核的昼夜节律计时特性集中在细胞自主转录反馈回路上。Brancaccio等人现在揭示了Gq-[Ca 2 +]i轴在视交叉上神经元回路水平编码昼夜节律时间。
The role of intracellular transcriptional/post-translational feedback loops (TTFL) within the circadian pacemaker of the suprachiasmatic nucleus (SCN) is well established. In contrast, contributions from G-coupled pathways and cytosolic rhythms to the intercellular control of SCN pacemaking are poorly understood. We therefore combined viral transduction of SCN slices with fluorescence/bioluminescence imaging to visualize GCaMP3-reported circadian oscillations of intracellular calcium [Ca2+]i alongside activation of Ca2+/cAMP-responsive elements. We phase-mapped them to the TTFL, in time and SCN space, and demonstrated their dependence upon G-coupled vasoactive intestinal peptide (VIP) signaling. Pharmacogenetic manipulation revealed the individual contributions of Gq, Gs, and Gi to cytosolic and TTFL circadian rhythms. Importantly, activation of Gq-dependent (but not Gs or Gi) pathways in a minority of neurons reprogrammed [Ca2+]i and TTFL rhythms across the entire SCN. This reprogramming was mediated by intrinsic VIPergic signaling, thus revealing a Gq/[Ca2+]i-VIP leitmotif and unanticipated plasticity within network encoding of SCN circadian time. SCN [Ca2+]i and TTFL circadian landscape phase-mapped by real-time imaging SCN network reprogrammed by recruitment of Gq-[Ca2+]i axis in a minority of neurons Selective Gq-mediated reprogramming mediated by intrinsic VIPergic signaling Internal structure and unanticipated plasticity of the SCN circadian network unveiled Unraveling the circadian timekeeping properties of the suprachiasmatic nucleus has focused upon cell-autonomous transcriptional feedback loops. Brancaccio et al. now reveal that a Gq-[Ca2+]i axis encodes circadian time at the level of the suprachiasmatic neuronal circuitry.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
影响因子：
5.3
作者：
Harrisingh, Marie C.;Wu, Ying;Nitabach, Michael N.
通讯作者：
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影响因子：
3.4
作者：
Hong, Jin Hee;Jeong, Byeongha;Lee, Kyoung J.
通讯作者：
Lee, Kyoung J.
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影响因子：
3.5
作者：
Butler MP;Silver R
通讯作者：
Silver R
影响因子：
16.6
作者：
Doi, Masao;Ishida, Atsushi;Miyake, Akiko;Sato, Miho;Komatsu, Rie;Yamazaki, Fumiyoshi;Kimura, Ikuo;Tsuchiya, Soken;Kori, Hiroshi;Seo, Kazuyuki;Yamaguchi, Yoshiaki;Matsuo, Masahiro;Fustin, Jean-Michel;Tanaka, Rina;Santo, Yasuko;Yamada, Hiroyuki;Takahashi, Yukari;Araki, Michihiro;Nakao, Kazuki;Aizawa, Shinichi;Kobayashi, Masaki;Obrietan, Karl;Tsujimoto, Gozoh;Okamura, Hitoshi
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Okamura, Hitoshi