A limited number of Caenorhabditis elegans genes are readily mutable to dominant, temperature-sensitive maternal-effect embryonic lethality.
A limited number of Caenorhabditis elegans genes are readily mutable to dominant, temperature-sensitive maternal-effect embryonic lethality.
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有限数量的秀丽隐杆线虫基因很容易突变为显性的、温度敏感的母体效应胚胎致死性。
作者：
Nancy L. Mitenko;J. R. Eisner;J. Swiston;P. Mains
显性功能获得性突变可以为基因功能的研究提供独特的见解。此外，功能获得性突变与功能丧失性等位基因不同，不会对遗传冗余位点的鉴定产生偏见。为了鉴定秀丽隐杆线虫胚胎发生过程中活跃的新的遗传功能，我们收集了一组显性温度敏感的母体效应胚胎致死突变。在之前的筛选中，我们分离出了八个这样的突变，分布在六个基因中。在本研究中，我们描述了8个新的显性突变，仅识别了3个额外的基因，总共在9个基因中发现了16个显性突变。因此，似乎有限数量的C。秀丽线虫基因以可感知的频率突变成这种表型。我们通过显性突变确定的基因中有五个具有功能丧失的等位基因。这些基因中的两个可能缺乏功能丧失表型，表明它们是非必需的，因此可能代表冗余位点。其他三个基因的功能丧失突变与隐性致死相关，表明非冗余。
Dominant gain-of-function mutations can give unique insights into the study of gene function. In addition, gain-of-function mutations, unlike loss-of-function alleles, are not biased against the identification of genetically redundant loci. To identify novel genetic functions active during Caenorhabditis elegans embryogenesis, we have collected a set of dominant temperature-sensitive maternal-effect embryonic lethal mutations. In a previous screen, we isolated eight such mutations, distributed among six genes. In the present study, we describe eight new dominant mutations that identify only three additional genes, yielding a total of 16 dominant mutations found in nine genes. Therefore, it appears that a limited number of C. elegans genes mutate to this phenotype at appreciable frequencies. Five of the genes that we identified by dominant mutations have loss-of-function alleles. Two of these genes may lack loss-of-function phenotypes, indicating that they are nonessential and so may represent redundant loci. Loss-of-function mutations of three other genes are associated with recessive lethality, indicating nonredundancy.
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影响秀丽隐杆线虫肌肉兴奋的显性突变分析。
影响因子：
3.3
作者：
Reiner,DJ;Weinshenker,D;Thomas,JH
通讯作者：
Thomas,JH
秀丽隐杆线虫 RNA 聚合酶 II 基因、ama-1 IV 和附近的必需基因。
影响因子：
3.3
作者：
Rogalski,TM;Riddle,DL
通讯作者：
Riddle,DL
影响早期秀丽隐杆线虫胚胎减数分裂和有丝分裂的突变。
影响因子：
3.3
作者：
Mains,PE;Kemphues,KJ;Sprunger,SA;Sulston,IA;Wood,WB
通讯作者：
Wood,WB
导致秀丽隐杆线虫胚胎死亡的显性母体效应突变。
影响因子：
3.3
作者：
Mains,PE;Sulston,IA;Wood,WB
通讯作者：
Wood,WB