High resolution X-ray micrography of live Candida albicans using laser plasma pulsed point X-ray sources.
High resolution X-ray micrography of live Candida albicans using laser plasma pulsed point X-ray sources.
复制标题
使用激光等离子体脉冲点 X 射线源对活白色念珠菌进行高分辨率 X 射线显微成像。
作者：
J. Rajyaguru;M. Kado;K. Nekula;M. Richardson;M. J. Muszynski
尽管由于可视化标本所需的准备工作而受到限制，电子显微镜仍然是最常用的亚细胞结构可视化方法，高分辨率 X 射线显微镜是一种相对较新的技术，仍在开发中，并且仅限于少数大型同步加速器 X 射线源。我们利用单次激光（纳秒）等离子体产生类似于同步加速器设施的 X 射线，对白色念珠菌的活细胞进行成像。调整发射光谱以实现富碳材料的最佳吸收。然后用原子力显微镜扫描光刻胶，得到微分 X 射线吸收图案。使用这项技术，样本成像时间为 90 分钟，我们在白色念珠菌细胞周围观察到了明显的 152.24 nm 厚一致环形结构（代表细胞壁），以及内部明显的“陨石坑”，其中一个直径为 570-90 nm，三个较小的陨石坑，每个直径为 400 nm。该技术在活的水合微生物样品和大分子的超微结构研究中的应用值得进一步探索。
Electron microscopy is still the most frequently used method for visualization of subcellular structures in spite of limitations due to the preparation required to visualize the specimen, High resolution X-ray microscopy is a relatively new technique, still under development and restricted to a few large synchrotron X-ray sources. We utilized a single-shot laser (nanosecond) plasma to generate X-rays similar to synchrotron facilities to image live cells of Candida albicans. The emission spectrum was tuned for optimal absorption by carbon-rich material. The photoresist was then scanned by an atomic force microscope to give a differential X-ray absorption pattern. Using this technique, with a sample image time of 90 min, we have visualized a distinct 152.24 nm thick consistent ring structure around cells of C albicans representing the cell wall, and distinct 'craters' inside, one of 570-90 nm diameter and three smaller ones, each 400 nm in diameter. This technique deserves further exploration concerning its application in the ultrastructural study of live, hydrated microbiological samples and of macromolecules.
使用新型 X 射线接触显微镜对巨噬细胞进行直接超微结构成像。
DOI：
10.1046/j.1525-1373.1999.d01-4.x
发表时间：
1999
期刊：
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
影响因子：
--
作者：
Kado,M;Richardson,M;Rajyaguru,JM;Muszynski,MJ;Friedman,H;Yamamoto,Y
通讯作者：
Yamamoto,Y
影响因子：
56.9
作者：
REES, WA;KELLER, RW;BUSTAMANTE, C
通讯作者：
BUSTAMANTE, C