Heat shock protein 10 of Chlamydophila pneumoniae induces proinflammatory cytokines through Toll-like receptor (TLR) 2 and TLR4 in human monocytes THP-1
Heat shock protein 10 of Chlamydophila pneumoniae induces proinflammatory cytokines through Toll-like receptor (TLR) 2 and TLR4 in human monocytes THP-1
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肺炎衣原体热休克蛋白 10 通过人单核细胞 THP-1 中的 Toll 样受体 (TLR) 2 和 TLR4 诱导促炎细胞因子
DOI：
10.1007/s11626-011-9441-4
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Chen, C.
中科院分区：
文献类型：
--
作者：
Zhou, Z.;Wu, Y.;Chen, L.;Liu, L.;Chen, H.;Li, Z.;Chen, C.
炎症反应是感染的第一线。以往的研究表明，肺炎衣原体热休克蛋白60存在于人类动脉粥样硬化中，在肺炎衣原体引起的慢性感染中起重要作用。在此，我们在体外研究了肺炎衣原体热休克蛋白10(HSP10)对THP-1细胞的影响以及Toll样受体(TLRs)在炎症反应过程中的作用。重组HSP10可剂量依赖性地诱导肿瘤坏死因子α、白介素6和白介素1β等促炎细胞因子的产生，加热和去蛋白处理可显著降低其促炎活性。用逆转录聚合酶链式反应和免疫荧光法检测培养细胞TLR4和TLR2的表达。用去内毒素蛋白刺激野生型(C3H/HEN)和TLR4缺陷小鼠(C3HEJ)的腹膜巨噬细胞。刺激后的细胞因子反应明显不同，这取决于TLR4的存在。抗TLR2或抗TLR4单抗可部分或显著阻断其对细胞因子表达的影响。因此，
Inflammatory response is the first line of infection. Previous studies have suggested that Chlamydophila pneumoniae heat shock protein (CHSP) 60 is present in human atheromata, and it plays an important role on the chronic infection elicited by C. pneumoniae. Here, we demonstrated in vitro the impact of heat shock protein 10 (HSP10) of C. pneumoniae on THP-1 cells and the role of Toll-like receptors (TLRs) in the procedures of inflammatory response. The production of proinflammatory cytokines, including tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, and IL-1beta were induced by recombinant HSP10 dose-dependently, and the proinflammatory activity of HSP10 was greatly reduced by heating and deproteinization treatment. The expression of TLR4 and TLR2 on the cultured cells were determined by reverse transcriptase-polymerase chain reaction and immunofluorescence. Peritoneal macrophages isolated from wild-type (C3H/HeN) and TLR4-deficient mice (C3H/HeJ) were respectively stimulated with endotoxin-free proteins. Cytokine responses after stimulation were significantly different, depending on the presence of TLR4. The effect on cytokine expression was blocked by anti-TLR2 or anti-TLR4 MAb partially or dramatically. Thus,
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免疫相关疾病中的热休克蛋白 10 (Hsp10)：一枚硬币，两面。
DOI：
--
发表时间：
2011
期刊：
Int J Biochem Mol Biol.
影响因子：
--
作者：
Hu L;Cai W;Liu J;Huang B
通讯作者：
Huang B
DOI：
10.1093/jac/44.2.145
发表时间：
1999-08
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
P. Cook
通讯作者：
P. Cook
影响因子：
4.6
作者：
S. Ragno;V. Winrow;Paolo Mascagni;P. Lucietto;F. D. Pierro;Christopher Morris;D. R. Blake
通讯作者：
S. Ragno;V. Winrow;Paolo Mascagni;P. Lucietto;F. D. Pierro;Christopher Morris;D. R. Blake
影响因子：
3.1
作者：
Liisa Törmäkangas;E. Markkula;K. Lounatmaa;M. Puolakkainen
通讯作者：
Liisa Törmäkangas;E. Markkula;K. Lounatmaa;M. Puolakkainen
影响因子：
6.9
作者：
N. Miyashita;K. Ouchi;Kozo Kawasaki;H. Komura;Y. Kawai;Y. Obase;Y. Kobashi;M. Oka
通讯作者：
N. Miyashita;K. Ouchi;Kozo Kawasaki;H. Komura;Y. Kawai;Y. Obase;Y. Kobashi;M. Oka