Transcriptome profiling identifies ABA mediated regulatory changes towards storage filling in developing seeds of castor bean (Ricinus communis L.).
Transcriptome profiling identifies ABA mediated regulatory changes towards storage filling in developing seeds of castor bean (Ricinus communis L.).
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DOI：
10.1186/2045-3701-4-33
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发表时间：
2014
影响因子：
7.5
通讯作者：
Liu A
中科院分区：
文献类型：
--
作者：
Chandrasekaran U;Xu W;Liu A
潜在的生物柴油植物蓖麻子（蓖麻）一直是生物能源研究的焦点，因为其基因组的可用性提高了全基因组研究的门槛，声称影响“基因组-表型组挑战”的进展。在这里，我们报告的应用植物激素阿坝作为一种外源因子，用于改善储存储备积累的重点与种子灌浆相关的途径的复杂的相互作用。应用外源阿坝处理后，我们用ELISA技术测定了体外培养的发育种子中阿坝水平的增加，并定量了处理种子中主要生物分子（包括总脂、糖和蛋白质）的含量。外源阿坝（10 μM）促进了可溶性糖含量的积累（6.3%）和总脂含量的沉积（4.9%）。为了阐明阿坝信号转导途径对种子灌浆的影响，我们利用Illumina RNA测序技术对差异表达基因进行了分析，共鉴定出2568个差异表达基因（1507个上调，1061个下调）。这些基因与糖代谢有关（如葡萄糖-6-磷酸、果糖-1，6-二磷酸、甘油-3-磷酸、丙酮酸激酶）、脂质生物合成（如ACS、ACBP、GPAT 2、GPAT 3、FAD 2、FAD 3、SAD 1和DGAT 1），贮藏蛋白合成（如SGP 1、锌指蛋白、RING H2蛋白、拟南芥素55和细胞色素P450）和阿坝生物合成（如NCED 1、NCED 3和β-胡萝卜素）。此外，我们通过半定量RT-PCR分析证实了RNA测序数据的有效性。综上所述，本研究中所示的基因和途径表达结果支持的代谢物测量为理解阿坝信号传导机制对种子贮藏填充提供了新的见解，也为促进油料作物功能基因组学提供了有用的信息，旨在利用蓖麻子农业和生物能源利用。
The potential biodiesel plant castor bean (Ricinus communis) has been in the limelight for bioenergy research due to the availability of its genome which raises the bar for genome-wide studies claiming advances that impact the “genome-phenome challenge”. Here we report the application of phytohormone ABA as an exogenous factor for the improvement of storage reserve accumulation with a focus on the complex interaction of pathways associated with seed filling. After the application of exogenous ABA treatments, we measured an increased ABA levels in the developing seeds cultured in vitro using the ELISA technique and quantified the content of major biomolecules (including total lipids, sugars and protein) in treated seeds. Exogenous ABA (10 μM) enhanced the accumulation of soluble sugar content (6.3%) followed by deposition of total lipid content (4.9 %). To elucidate the possible ABA signal transduction pathways towards overall seed filling, we studied the differential gene expression analysis using Illumina RNA-Sequencing technology, resulting in 2568 (1507-up/1061-down regulated) differentially expressed genes were identified. These genes were involved in sugar metabolism (such as glucose-6-phosphate, fructose 1,6 bis-phosphate, glycerol-3-phosphate, pyruvate kinase), lipid biosynthesis (such as ACS, ACBP, GPAT2, GPAT3, FAD2, FAD3, SAD1 and DGAT1), storage proteins synthesis (such as SGP1, zinc finger protein, RING H2 protein, nodulin 55 and cytochrome P450), and ABA biosynthesis (such as NCED1, NCED3 and beta carotene). Further, we confirmed the validation of RNA-Sequencing data by Semi-quantitative RT-PCR analysis. Taken together, metabolite measurements supported by genes and pathway expression results indicated in this study provide new insights to understand the ABA signaling mechanism towards seed storage filling and also contribute useful information for facilitating oilseed crop functional genomics on an aim for utilizing castor bean agricultural and bioenergy use.
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