Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii.
Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii.
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编码SAG1/SAG3与霍乱毒素A2/B亚基的复合DNA疫苗作为遗传佐剂保护BALB/c小鼠免受弓形虫感染
DOI：
10.1186/1756-3305-6-63
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发表时间：
2013-03-13
影响因子：
3.2
通讯作者：
He S
中科院分区：
文献类型：
--
作者：
Cong H;Zhang M;Xin Q;Wang Z;Li Y;Zhao Q;Zhou H;He S
背景：细胞内寄生虫，如T。弓形虫由于其生命周期的复杂性而呈现多种抗原。复合DNA疫苗为弓形虫病的治疗带来了新的途径和希望。本研究以T.方法：分别用PBS、pcDNA3.1、pSAG 1、pSAG 1/SAG 3和pSAG 1/SAG 3-CTXA 2/B免疫BALB/c小鼠3次。通过ELISA检测免疫小鼠的IgG抗体以及IFN-γ和IL-4的产生。DNA合成法检测T细胞增殖活性，流式细胞仪检测脾细胞淋巴细胞亚群。结果：pSAG 1/SAG 3免疫组小鼠的IgG抗体产生、抗原特异性淋巴细胞增殖和脾细胞IFN-γ产生均显著高于pSAG 1免疫组（P<0.05）; CTXA 2/B的引入进一步增强了Th 1细胞介导的免疫，具有更高水平的IFN-γ、淋巴细胞增殖活性和CD 8 + T细胞百分比。当用致死剂量的T.对照组（PBS组和空质粒组）均在6天内死亡。用pSAG 1免疫的小鼠在8天内死亡。而pSAG 1/SAG 3和pSAG 1/SAG 3-CTXA 2/B免疫小鼠的存活率分别为20%和40%。弓形虫抗原SAG 1、SAG 3与弓形虫CTXA 2/B基因结合，可有效增强免疫小鼠的体液免疫和细胞免疫应答，延长免疫小鼠的存活时间，是一种很有前途的弓形虫DNA疫苗候选者。
Background:Intracellular parasites, such as T. gondii, present a plurality of antigens because of the complexity of its life cycle. Compound DNA vaccines bring a new approach and hope for the treatment of toxoplasmosis. In this study, a DNA vaccine encoding two major surface antigens SAG1, SAG3 from T. gondii, with A2/B subunit of cholera toxin as a genetic adjuvant was constructed.Methods:BALB/c mice were immunized intramuscularly with PBS, pcDNA3.1, pSAG1, pSAG1/SAG3 and pSAG1/SAG3-CTXA2/B three times separately. Immunized mice were tested for IgG antibody and IFN-γ and IL-4 production by ELISA. The proliferation of T cells was measured by DNA synthesis assay and the lymphocyte subsets of spleen cells by flow cytometry. All the immunized mice were challenged with 103 highly virulent RH tachyzoites of Toxoplasma gondii intraperitoneally and the survival times were recorded.Results:An enhanced production of IgG antibodies, antigen-specific lymphocyte proliferation and IFN-γ production from splenic cells were induced in mice immunized with pSAG1/SAG3 compared to mice immunized with pSAG1 (P<0.05). Introduction of CTXA2/B further enhanced the Th1 cell-mediated immunity with higher levels of IFN-γ, lymphocyte proliferation activity and percentage of CD8+ T-cells. When challenged with lethal doses of T. gondii (1 × 103), all control mice (PBS and empty plasmid group) died within 6 days. Mice immunized with pSAG1 died within 8 days. While 20% and 40% survival rate were achieved from mice immunized with pSAG1/SAG3 and pSAG1/SAG3-CTXA2/B.Conclusions:This study indicates the compound DNA vaccine encoding T. gondii antigens SAG1, SAG3 with CTXA2/B gene was a promising DNA vaccine candidate against toxoplasmosis, which could effectively enhance the humoral and cellular immune response and prolong survival time in vaccinated mice.
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影响因子：
--
作者：
Wang, Pei-Yuan;Yuan, Zi-Guo;Zhu, Xing-Quan
通讯作者：
Zhu, Xing-Quan
影响因子：
2.2
作者：
Angus, CW;Klivington, D;Kovacs, JA
通讯作者：
Kovacs, JA
影响因子：
2.4
作者：
BUXTON, D;INNES, EA
通讯作者：
INNES, EA
影响因子：
2.3
作者：
Wang, XS;Claflin, J;Suzuki, Y
通讯作者：
Suzuki, Y
影响因子：
3.1
作者：
Eriksson, K;Fredriksson, M;Holmgren, J
通讯作者：
Holmgren, J