Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo.
Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo.
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DOI：
10.1016/j.ydbio.2011.12.024
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发表时间：
2012-03-01
影响因子：
2.7
通讯作者：
Yaguchi S
中科院分区：
文献类型：
--
作者：
Yaguchi J;Angerer LM;Inaba K;Yaguchi S
血清素能神经元在海胆胚胎的神经源性动物板外胚层中分化。尽管在对基因组进行测序后，鉴定出了许多编码在该区域表达的转录因子的基因，但控制海胆胚胎中这些神经元的规范或分化的调节机制尚不清楚。在这里，我们报告锌指同源盒（zfhx1/z81）在血清素能神经前体细胞中表达，使用血清素能神经标记物色氨酸5-羟化酶（tph）进行双原位杂交筛选，色氨酸5-羟化酶（tph）编码血清素能神经元分化所需的血清素合酶。 zfhx1/z81 在原肠胚阶段开始在前神经外胚层的单个细胞中表达，其中一些细胞也表达 delta。随着神经分化以 tph 表达开始，zfhx1/z81 表达逐渐消失。当Zfhx1/Z81的翻译被吗啉注射阻断时，胚胎在动物板的细胞中既不表达tph也不表达神经标记突触结合蛋白B，并且血清素能神经元不分化。相比之下，Zfhx1/Z81 变形体确实表达 fez，这是另一种神经前体标记，它似乎在血清素能神经元的规范/分化的初始阶段发挥作用。此外，zfhx1/z81是动物板中被Nodal和Delta-Notch等抗神经信号抑制的靶标之一。我们得出结论，Zfhx1/Z81 在个体前神经前体的规范过程中发挥作用，并促进 tph 和突触结合蛋白 B 的表达，这是血清素能神经元分化所需的。
Serotonergic neurons differentiate in the neurogenic animal plate ectoderm of the sea urchin embryo. The regulatory mechanisms that control the specification or differentiation of these neurons in the sea urchin embryo are not yet understood, although, after the genome was sequenced, many genes encoding transcription factors expressed in this region were identified. Here, we report that zinc finger homeobox (zfhx1/z81) is expressed in serotonergic neural precursor cells, using double in situ hybridization screening with a serotonergic neural marker, tryptophan 5-hydroxylase (tph) encoding a serotonin synthase that is required for the differentiation of serotonergic neurons. zfhx1/z81 begins to be expressed at gastrula stage in individual cells in the anterior neuroectoderm, some of which also express delta. zfhx1/z81 expression gradually disappears as neural differentiation begins with tph expression. When the translation of Zfhx1/Z81 is blocked by morpholino injection, embryos express neither tph nor the neural marker synaptotagminB in cells of the animal plate, and serotonergic neurons do not differentiate. In contrast, Zfhx1/Z81 morphants do express fez, another neural precursor marker, which appears to function in the initial phase of specification/differentiation of serotonergic neurons. In addition, zfhx1/z81 is one of the targets suppressed in the animal plate by anti-neural signals such as Nodal as well as Delta-Notch. We conclude that Zfhx1/Z81 functions during the specification of individual anterior neural precursors and promotes the expression of tph and synaptotagminB, required for the differentiation of serotonergic neurons.
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影响因子：
9.8
作者：
Lapraz F;Besnardeau L;Lepage T
通讯作者：
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影响因子：
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GENETTA, T;RUEZINSKY, D;KADESCH, T
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KADESCH, T
影响因子：
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作者：
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Lepage, Thierry
影响因子：
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作者：
Bradham, Cynthia A.;Oikonomou, Catherine;Kuehn, Alexander;Core, Amanda B.;Modell, Joshua W.;McClay, David R.;Poustka, Albert J.
通讯作者：
Poustka, Albert J.
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通讯作者：
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