Plasma membrane permeabilization by trains of ultrashort electric pulses.
Plasma membrane permeabilization by trains of ultrashort electric pulses.
复制标题
DOI：
10.1016/j.bioelechem.2010.01.001
复制
发表时间：
2010-08
影响因子：
5
通讯作者：
Pakhomov, Andrei G.
中科院分区：
文献类型：
--
作者：
Ibey, Bennett L.;Mixon, Dustin G.;Payne, Jason A.;Bowman, Angela;Sickendick, Karl;Wilmink, Gerald J.;Roach, W. Patrick;Pakhomov, Andrei G.
超短电脉冲（USEP）引起细胞膜电导率的长期增加，单次USEP增加细胞膜电导率与吸收剂量成正比，阈值约为10 mJ/g。本研究将膜渗透效应的量化扩展到多个USEP，并采用了更准确的方案，将USEP效应确定为单个细胞暴露后和暴露前电导值之间的差异（Δg）。我们证明了Δg可以通过在恒定的e场中增加脉冲数，或者在恒定的脉冲数下增加e场来增加。对于60 ns脉冲，通过施加100个脉冲或更长的脉冲串，将单个脉冲的6 kV/cm的e场阈值降低到1.7 kV/cm以下。然而，与单脉冲暴露相比，e场阈值的降低仅以更高的AD为代价。此外，多个脉冲的影响并不能完全由AD决定，这表明在序列中被第一个脉冲渗透的细胞对随后的脉冲变得不那么脆弱。这一解释得到了一个模型的证实，该模型将多脉冲暴露视为一系列单脉冲暴露，并假设细胞对USEP的敏感性呈指数下降，因为在训练过程中，每次脉冲后Δg增加。
Ultrashort electric pulses (USEP) cause long-lasting increase of cell membrane electrical conductance, and that a single USEP increased cell membrane electrical conductance proportionally to the absorbed dose (AD) with a threshold of about 10 mJ/g. The present study extends quantification of the membrane permeabilization effect to multiple USEP and employed a more accurate protocol that identified USEP effect as the difference between post- and pre-exposure conductance values (Δg) in individual cells. We showed that Δg can be increased by either increasing the number of pulses at a constant E-field, or by increasing the E-field at a constant number of pulses. For 60-ns pulses, an E-field threshold of 6 kV/cm for a single pulse was lowered to less than 1.7 kV/cm by applying 100-pulse or longer trains. However, the reduction of the E-field threshold was only achieved at the expense of a higher AD compared to a single pulse exposure. Furthermore, the effect of multiple pulses was not fully determined by AD, suggesting that cells permeabilized by the first pulse(s) in the train become less vulnerable to subsequent pulses. This explanation was corroborated by a model that treated multiple-pulse exposures as a series of single-pulse exposures and assumed an exponential decline of cell susceptibility to USEP as Δg increased after each pulse during the course of the train.
登录
查看更多内容
DOI：
10.1016/j.mrgentox.2003.08.006
发表时间：
2003-12-09
影响因子：
1.9
作者：
Stacey, M;Stickley, J;Buescher, S
通讯作者：
Buescher, S
影响因子：
1.9
作者：
Schoenbach, KH;Beebe, SJ;Buescher, ES
通讯作者：
Buescher, ES
影响因子：
--
作者：
Vernier, P. Thomas;Sun, Yinghua;Gundersen, Martin A.
通讯作者：
Gundersen, Martin A.
影响因子：
1.9
作者：
Pakhomov, Andrei G.;Kolb, Juergen F.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
3.9
作者：
Pakhomov, Andrei G.;Shevin, Rachael;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.