Competition between Burkholderia pseudomallei and B. thailandensis.
Competition between Burkholderia pseudomallei and B. thailandensis.
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DOI：
10.1186/s12866-015-0395-7
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发表时间：
2015-03-03
期刊：
影响因子：
4.2
通讯作者：
Chantratita N
中科院分区：
文献类型：
--
作者：
Ngamdee W;Tandhavanant S;Wikraiphat C;Reamtong O;Wuthiekanun V;Salje J;Low DA;Peacock SJ;Chantratita N
假鼻疽伯克霍尔德氏菌是一种革兰氏阴性细菌，会导致类鼻疽病，这是一种在热带国家经常致命的疾病。泰兰伯克霍尔德氏菌是一种无毒但亲缘关系密切的物种。这两个物种都是土壤腐生植物，但几乎从未被隔离在一起。我们发现假鼻疽杆菌影响泰兰假单胞菌生长的两种机制。首先，我们发现了6个不同的假鼻疽杆菌菌株在Lb琼脂平板上对泰兰杆菌的生长有抑制作用。其次，我们的结果表明，55%的假鼻疽分离株产生一种分泌性化合物，抑制泰兰杆菌的运动，但不抑制其生存能力。分析表明，该活性化合物是一种pH敏感、热不稳定的化合物，可能是一种蛋白质，可能会影响鞭毛的加工或促进其降解。对细菌序列类型(STS)的分析表明，这与运动抑制之间存在关联。该活性化合物是假鼻疽杆菌在生长稳定期产生的。综上所述，我们的结果表明，假鼻疽杆菌对其近亲泰兰杆菌的生长和运动都有抑制作用。后一种现象似乎是通过一种以前未报道的涉及鞭毛加工或降解的机制发生的。本文的在线版本(doi：10.1186/s12866-0150395-7)包含补充材料，授权用户可以使用。
Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, an often fatal disease in tropical countries. Burkholderia thailandensis is a non-virulent but closely related species. Both species are soil saprophytes but are almost never isolated together. We identified two mechanisms by which B. pseudomallei affects the growth of B. thailandensis. First, we found that six different isolates of B. pseudomallei inhibited the growth of B. thailandensis on LB agar plates. Second, our results indicated that 55% of isolated strains of B. pseudomallei produced a secreted compound that inhibited the motility but not the viability of B. thailandensis. Analysis showed that the active compound was a pH-sensitive and heat-labile compound, likely a protein, which may affect flagella processing or facilitate their degradation. Analysis of bacterial sequence types (STs) demonstrated an association between this and motility inhibition. The active compound was produced from B. pseudomallei during the stationary growth phase. Taken together, our results indicate that B. pseudomallei inhibits both the growth and motility of its close relative B. thailandensis. The latter phenomenon appears to occur via a previously unreported mechanism involving flagellar processing or degradation. The online version of this article (doi:10.1186/s12866-015-0395-7) contains supplementary material, which is available to authorized users.
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影响因子：
9.4
作者：
Vesaratchavest, Mongkol;Tumapa, Sarinna;Peacock, Sharon J.
通讯作者：
Peacock, Sharon J.
影响因子：
6.7
作者：
Schwarz S;West TE;Boyer F;Chiang WC;Carl MA;Hood RD;Rohmer L;Tolker-Nielsen T;Skerrett SJ;Mougous JD
通讯作者：
Mougous JD
影响因子：
4.5
作者：
Anderson MS;Garcia EC;Cotter PA
通讯作者：
Cotter PA
影响因子：
3.2
作者：
DeShazer, D;Brett, PJ;Woods, DE
通讯作者：
Woods, DE
影响因子：
4.2
作者：
Brett, PJ;Deshazer, D;Woods, DE
通讯作者：
Woods, DE