Control of O-Glycan Branch Formation
Control of O-Glycan Branch Formation
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O-聚糖分支形成的控制
DOI：
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发表时间：
1999
影响因子：
4.8
通讯作者：
H. Clausen
中科院分区：
文献类型：
--
作者：
T. Schwientek;M. Nomoto;S. Levery;G. Merkx;A. V. van Kessel;E. Bennett;M. Hollingsworth;H. Clausen
通过对表达序列标签的BLAST分析，鉴定了一种新的人尿苷二磷酸葡萄糖NAc：Gal/GlcNAcβ1-3GalNAcα β1，6 GlcNAc-转移酶，命名为C2/4GnT。C2/4GnT基因编码一种II型跨膜蛋白，与人C2 GnT和IGnT基因序列相似。分泌型C2/4GnT在昆虫细胞中的表达结果表明，该基因产物具有UDP-N-乙酰-α-d-葡糖胺：受体β 1，6-N-乙酰葡糖胺转移酶（β1，6 GlcNAc-transferase，β1，6 GlcNAc-transferase）活性。底物特异性分析显示，该酶催化核心2和核心4型的O-聚糖分支形成。用核3-对硝基苯基形成的产物的NMR分析证实了产物核4-对硝基苯基。C2/4GnT基因编码区位于染色体15q21.3，为单外显子。北方分析显示C2/4GnT主要在结肠、肾脏、胰腺和小肠中限制性表达。在脑、心脏、肝脏、卵巢、胎盘、脾脏、胸腺和外周血白细胞中均未检测到C2/4GnT的表达。核心20-聚糖的表达与细胞分化过程和癌症相关。结果证实了预测的在核心2和核心4 O-聚糖分支形成中起作用的β1，6 GlcNAc-转移酶的存在。能够形成核心2 O-聚糖的β1，6 GlcNAc-转移酶的冗余对于理解导致细胞发育和恶性转化期间核心2分支的特异性变化的机制是重要的。
A novel human UDP-GlcNAc:Gal/GlcNAcβ1–3GalNAcα β1,6GlcNAc-transferase, designated C2/4GnT, was identified by BLAST analysis of expressed sequence tags. The sequence of C2/4GnT encoded a putative type II transmembrane protein with significant sequence similarity to human C2GnT and IGnT. Expression of the secreted form of C2/4GnT in insect cells showed that the gene product had UDP-N-acetyl-α-d-glucosamine:acceptor β1,6-N-acetylglucosaminyltransferase (β1,6GlcNAc-transferase) activity. Analysis of substrate specificity revealed that the enzyme catalyzed O-glycan branch formation of the core 2 and core 4 type. NMR analyses of the product formed with core 3-para-nitrophenyl confirmed the product core 4-para-nitrophenyl. The coding region of C2/4GnT was contained in a single exon and located to chromosome 15q21.3. Northern analysis revealed a restricted expression pattern of C2/4GnT mainly in colon, kidney, pancreas, and small intestine. No expression of C2/4GnT was detected in brain, heart, liver, ovary, placenta, spleen, thymus, and peripheral blood leukocytes. The expression of core 2O-glycans has been correlated with cell differentiation processes and cancer. The results confirm the predicted existence of a β1,6GlcNAc-transferase that functions in both core 2 and core 4O-glycan branch formation. The redundancy in β1,6GlcNAc-transferases capable of forming core 2O-glycans is important for understanding the mechanisms leading to specific changes in core 2 branching during cell development and malignant transformation.
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编码 N-乙酰葡糖胺基转移酶 V 的 cDNA 的分离、表征和表达。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shoreibah,M;Perng,GS;Adler,B;Weinstein,J;Basu,R;Cupples,R;Wen,D;Browne,JK;Buckhaults,P;Fregien,N;Pierce,M
通讯作者：
Pierce,M
DOI：
10.1016/s0021-9258(18)68157-8
发表时间：
1988-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Piller;V. Piller;R. Fox;Minoru Fukuda
通讯作者：
F. Piller;V. Piller;R. Fox;Minoru Fukuda
影响因子：
11.2
作者：
M. Fukuda
通讯作者：
M. Fukuda
影响因子：
4.3
作者：
YANG, JM;BYRD, JC;BROCKHAUSEN, I
通讯作者：
BROCKHAUSEN, I
DOI：
--
发表时间：
1989-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Paulson;Karen J. ColleyS
通讯作者：
J. Paulson;Karen J. ColleyS