CXCL1 gene silencing inhibits HGC803 cell migration and invasion and acts as an independent prognostic factor for poor survival in gastric cancer.
CXCL1 gene silencing inhibits HGC803 cell migration and invasion and acts as an independent prognostic factor for poor survival in gastric cancer.
复制标题
CXCL1基因沉默抑制HGC803细胞迁移和侵袭，并作为胃癌生存不良的独立预后因素。
DOI：
10.3892/mmr.2016.5843
复制
发表时间：
2016-11
影响因子：
3.4
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Wang L;Zhang C;Xu J;Wu H;Peng J;Cai S;He Y
趋化因子（C-X-C基序）配体1 （CXCL1）在恶性肿瘤的发生发展中起重要作用。本研究旨在探讨CXCL1表达在胃癌患者中促进淋巴结转移的作用。采用人胃癌细胞系检测CXCL1的表达。采用伤口愈合实验和Transwell侵袭实验分别检测HGC803细胞迁移和细胞侵袭。本研究纳入2007 - 2008年在中国广州中山大学第一附属医院行根治性胃切除术并淋巴结清扫术的患者100例。免疫组化（IHC）检测CXCL1的表达和淋巴管密度（LMVD），并探讨其与临床病理特征和预后的关系。采用Cox生存回归分析分析患者总生存期。结果表明，CXCL1蛋白在所有胃癌细胞系中均有表达。CXCL1基因的沉默降低了HGC803细胞的迁移和侵袭能力。41例（41%）患者通过免疫组化检测到CXCL1蛋白表达，这些患者与晚期肿瘤-淋巴结转移（TNM）分期、LMVD、肿瘤分化和生存不良相关。LMVD与TNM分期、肿瘤大小、肿瘤分化及不良生存率呈正相关。此外，在Cox生存回归分析中，TNM分期、肿瘤分化和CXCL1是独立的预后因素。CXCL1基因的沉默抑制HGC803细胞的迁移和侵袭。CXCL1阳性表达与胃癌患者生存不良相关，CXCL1是胃癌的独立预后因素。
Chemokine (C-X-C motif) ligand 1 (CXCL1) is essential in oncogenesis and development of malignant tumors. The present study aimed to investigate CXCL1 expression in promoting lymph node metastasis in gastric cancer patients. Human gastric cancer cell lines were employed to detect CXCL1 expression. HGC803 cell migration and cell invasion were detected using a wound healing assay and Transwell invasion assay, respectively. A total of 100 patients who underwent radical gastric resection with lymph node dissection in the First Affiliated Hospital of Sun Yat-Sen University (Guangzhou, China) between 2007 and 2008 were included. Expression of CXCL1 and lymphatic vessel density (LMVD) was determined by using immunohistochemistry (IHC), and their association with clinicopathological features and prognosis was investigated. Cox survival regression analysis was used to analyze overall survival of patients. Results indicated that CXCL1 protein was expressed in all of investigated gastric cancer cell lines. Silencing of the CXCL1 gene reduced migratory and invasive ability of HGC803 cells. CXCL1 protein expression was detected by IHC in 41 patients (41%), these were associated with advanced tumor-node-metastasis (TNM) stage, LMVD, tumor differentiation and poor survival. LMVD was positively correlated with advanced TNM stage, size of tumor, tumor differentiation and poor survival rate. Furthermore, it was observed that TNM stage, tumor differentiation and CXCL1 were independent prognostic factors in the Cox survival regression analysis. Silencing of the CXCL1 gene inhibits HGC803 cell migration and invasion. The positive expression of CXCL1 is correlated with poor survival of gastric cancer patients and CXCL1 is an independent prognostic factor for gastric cancer.
登录
查看更多内容
影响因子：
4.6
作者：
Eck, M;Schmausser, B;Müller-Hermelink, HK
通讯作者：
Müller-Hermelink, HK
影响因子：
4.8
作者：
Ravindran, Aishwarya;Sawant, Kirti V.;Rajarathnam, Krishna
通讯作者：
Rajarathnam, Krishna
影响因子：
6.4
作者：
Cheng, Wan-Li;Wan, Chia-Siu;Lin, Kwang-Huei
通讯作者：
Lin, Kwang-Huei
影响因子：
11.5
作者：
Bernal, Carolina;Aguayo, Francisco;Corvalan, Alejandro H.
通讯作者：
Corvalan, Alejandro H.
影响因子：
11.5
作者：
Kawanishi, Hiroaki;Matsui, Yoshiyuki;Ogawa, Osamu
通讯作者：
Ogawa, Osamu