Macrophages improve survival, proliferation and migration of engrafted myogenic precursor cells into MDX skeletal muscle.
Macrophages improve survival, proliferation and migration of engrafted myogenic precursor cells into MDX skeletal muscle.
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DOI：
10.1371/journal.pone.0046698
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chazaud B
中科院分区：
文献类型：
--
作者：
Lesault PF;Theret M;Magnan M;Cuvellier S;Niu Y;Gherardi RK;Tremblay JP;Hittinger L;Chazaud B
肌前体细胞移植对治疗局灶性骨骼肌疾病具有重要意义。然而，细胞移植的主要限制是注射细胞的存活、扩增和迁移差。移植成肌细胞的大量和早期死亡还没有完全理解，尽管已经提出了几种机制。为改善他们的生存或迁移，已经进行了各种尝试。考虑到肌肉再生与巨噬细胞的存在有关，巨噬细胞有助于通过清洁碎片并以顺序的方式向成肌细胞提供营养因子来修复肌肉，我们试图在目前的工作中通过共注射巨噬细胞来改善成肌细胞移植。目前的数据显示，在移植后5天内，巨噬细胞有效地改善了：i）通过限制成肌细胞的大量死亡而提高了成肌细胞的存活，ii）组织内的成肌细胞扩增和iii）营养不良肌肉中的成肌细胞迁移。体外分析证实了这一点，表明巨噬细胞刺激成肌细胞粘附和迁移。结果，移植后一个月，巨噬细胞增加了成肌细胞对宿主肌纤维再生的贡献。总之，这些数据表明，巨噬细胞在成肌细胞移植到骨骼肌的早期步骤中是有益的，表明共注射这些基质细胞可用作提高实质细胞移植效率的辅助物。
Transplantation of muscle precursor cells is of therapeutic interest for focal skeletal muscular diseases. However, major limitations of cell transplantation are the poor survival, expansion and migration of the injected cells. The massive and early death of transplanted myoblasts is not fully understood although several mechanisms have been suggested. Various attempts have been made to improve their survival or migration. Taking into account that muscle regeneration is associated with the presence of macrophages, which are helpful in repairing the muscle by both cleansing the debris and deliver trophic cues to myoblasts in a sequential way, we attempted in the present work to improve myoblast transplantation by coinjecting macrophages. The present data showed that in the 5 days following the transplantation, macrophages efficiently improved: i) myoblast survival by limiting their massive death, ii) myoblast expansion within the tissue and iii) myoblast migration in the dystrophic muscle. This was confirmed by in vitro analyses showing that macrophages stimulated myoblast adhesion and migration. As a result, myoblast contribution to regenerating host myofibres was increased by macrophages one month after transplantation. Altogether, these data demonstrate that macrophages are beneficial during the early steps of myoblast transplantation into skeletal muscle, showing that coinjecting these stromal cells may be used as a helper to improve the efficiency of parenchymal cell engraftment.
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影响因子：
2.8
作者：
Lescaudron, L;Peltékian, E;Parrish, E
通讯作者：
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影响因子：
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Bouchentouf, M;Benabdallah, BF;Tremblay, JP
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作者：
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通讯作者：
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