Intercellular and intracellular colonization of Azorhizobium caulinodans in Arabidopsis thaliana under nitrogen deficient conditions
Intercellular and intracellular colonization of Azorhizobium caulinodans in Arabidopsis thaliana under nitrogen deficient conditions
复制标题
缺氮条件下拟南芥中固氮根瘤菌 (Azorhizobium caulinodans) 的细胞间和细胞内定殖
DOI：
10.1080/00380768.2020.1842139
复制
发表时间：
2020
影响因子：
2
通讯作者：
Aono Toshihiro
中科院分区：
文献类型：
--
作者：
Kurumisawa Keigo;Ishizuna Fumiko;Matsuoka Jun-Ichi;Ogawa Tetsuhiro;Morohashi Kengo;Aono Toshihiro
Azorhizobium caulinodansORS571是热带豆科植物长喙田菁（Sesbania rostrata）的一种微共生菌。这种细菌在宿主植物的茎和根上诱导结瘤，并将氮固定在这些结瘤内的宿主细胞中。它也是一些非豆科植物如拟南芥、水稻、小麦和番茄的内生细菌。已知该细菌定殖于A. thalianaplants，但这些观察不是在完全无氮的条件下进行的。在本研究中，A.拟南芥植株接种A. caulinodan在无氮培养基上，发现A.茎节瘤促进了A.但这种促进作用不是由固氮作用引起的。详细的观察表明，A.茎节线虫不仅以细胞间和细胞内的方式侵染A.除此之外，还有一些植物。在叶片中，不仅在叶肉细胞的气孔和细胞间隙中，而且在保卫细胞和叶肉细胞中也经常观察到侵染。有趣的是，叶绿体也被A.茎节类在花朵中，A。在花粉细胞、柱头和花柱中心以及子房胚珠周围的空间中均发现有茎节。这表明A.茎生雄蕊通过花粉管使子房内部受精。综合来看，可以说这种细菌感染A的可能性更大。在氮素充足条件下，拟南芥植株的氮素利用率比氮素缺乏条件下高。本文的研究结果被认为是使用A的基础。茎节素接种用于在贫营养条件下增强植物生长。
Azorhizobium caulinodansORS571 is a microsymbiont of the tropical legumeSesbania rostrata. This bacterium induces nodules on the stems and roots of host plants, and fixes nitrogen in the host cells within such nodules. It is also an endophytic bacterium of some non-leguminous plants, such asArabidopsis thaliana, rice, wheat, and tomato. It is known that this bacterium colonizes in the intercellular spaces and xylems of the roots ofA. thalianaplants, but these observations were not conducted under completely nitrogen-free conditions. In this study, in whichA. thalianaplants were inoculated withA. caulinodanson nitrogen-free medium, it was found thatA. caulinodanspromoted the growth of theA. thalianaplants, however, the promotion was not caused by nitrogen fixation. Detailed observations revealed thatA. caulinodansintercellularly and intracellularly infected not only the roots but also almost the whole bodies of theA. thalianaplants, except for the stems. In the leaves, infection was frequently observed not only in the stomata and intercellular spaces of the mesophyll cells, but also in the guard cells and mesophyll cells. Interestingly, chloroplasts were also infected withA. caulinodans. In the flowers,A. caulinodanswas detected in the pollen cells, in the stigma and the center of the style, and in the space around the ovule in the ovary. This suggests thatA. caulinodanscolonizes the inside of ovaries via pollen tubes. Taken together, it can be said that this bacterium is more likely to infectA. thalianaplants in nitrogen deficient conditions than in nitrogen sufficient conditions. The findings herein are thought to be the basis for the use ofA. caulinodansinoculation for the enhancement of plant growth in oligotrophic conditions.
登录
查看更多内容
DOI：
10.1098/rspb.2000.0973
发表时间：
2000-01-22
影响因子：
4.7
作者：
Gopalaswamy, G;Kannaiyan, S;Cocking, EC
通讯作者：
Cocking, EC
影响因子：
3.5
作者：
D'Haeze, W;Gao, MS;Holsters, M
通讯作者：
Holsters, M
影响因子：
4.9
作者：
Gough, G;Vasse, J;Denarie, J
通讯作者：
Denarie, J
影响因子：
3.5
作者：
Stone, PJ;O'Callaghan, KJ;Cocking, EC
通讯作者：
Cocking, EC
带状螺旋线杆菌 47 和 116 中 R 体合成和组装的遗传决定因素的表征
影响因子：
3.2
作者：
D. Heruth;F. R. Pond;J. Dilts;R. L. Quackenbush
通讯作者：
R. L. Quackenbush