Identification of FBXO25-interacting proteins using an integrated proteomics approach.
Identification of FBXO25-interacting proteins using an integrated proteomics approach.
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DOI：
10.1002/pmic.200900419
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发表时间：
2010-08
期刊：
影响因子：
3.4
通讯作者：
Gomes, Marcelo Damario
中科院分区：
文献类型：
--
作者：
Teixeira, Felipe R.;Yokoo, Sami;Gartner, Carlos A.;Manfiolli, Adriana O.;Baqui, Munira M. A.;Assmann, Eliana M.;Maragno, Ana Leticia G. C.;Yu, Huijun;de Lanerolle, Primal;Kobarg, Joerg;Gygi, Steven P.;Gomes, Marcelo Damario
FBXO 25是68种人类F-box蛋白之一，作为泛素连接酶家族的特异性因子，该家族由Skp 1、Rbx 1、Cullin 1和F-box蛋白（SCF 1）组成，参与靶向蛋白质以破坏泛素蛋白酶体系统。我们最近报道，FBXO 25蛋白积累在新的亚核结构命名为FBXO 25相关的核结构域（FANDs）。结合两步亲和纯化，然后质谱与经典的双杂交筛选，我们确定了132个新的潜在FBXO 25相互作用的合作伙伴。鉴定的蛋白质之一β-肌动蛋白通过其N-末端与FBXO 25物理相互作用，并富集在FBXO 25核隔室中。肌动蛋白聚合的抑制剂促进FANDs的显着中断，表明它们是受细胞核中肌动蛋白组织状态影响的隔室。此外，FBXO 25抗体在体外干扰RNA聚合酶II转录。我们的研究结果为理解这种新的隔室及其核功能开辟了新的视角。
FBXO25 is one of 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of Skp1, Rbx1, Cullin1 and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome system. We recently reported that the FBXO25 protein accumulates in novel subnuclear structures named FBXO25-associated nuclear domains (FANDs). Combining two-step affinity purification followed by mass spectrometry with a classical two-hybrid screen, we identified 132 novel potential FBXO25 interacting partners. One of the identified proteins, β-actin, physically interacts through its N-terminus with FBXO25 and is enriched in the FBXO25 nuclear compartments. Inhibitors of actin polymerization promote a significant disruption of FANDs, indicating that they are compartments influenced by the organizational state of actin in the nucleus. Furthermore, FBXO25 antibodies interfered with RNA polymerase II transcription in vitro. Our results open new perspectives for the understanding of this novel compartment and its nuclear functions.
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影响因子：
64.5
作者：
Duda, David M.;Borg, Laura A.;Scott, Daniel C.;Hunt, Harold W.;Hammel, Michal;Schulman, Brenda A.
通讯作者：
Schulman, Brenda A.
影响因子：
3.3
作者：
Manfiolli, Adriana O.;Maragno, Ana Leticia G. C.;Gomes, Marcelo D.
通讯作者：
Gomes, Marcelo D.
DOI：
10.1083/jcb.200512083
发表时间：
2006-03-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Percipalle P;Visa N
通讯作者：
Visa N
DOI：
10.1016/j.bbagen.2006.03.020
发表时间：
2006-06-01
影响因子：
3
作者：
Maragno, Ana Leticia G. C.;Baqui, Munira M. A.;Gomes, Marcelo D.
通讯作者：
Gomes, Marcelo D.
影响因子：
16
作者：
Liu, JD;Furukawa, M;Xiong, Y
通讯作者：
Xiong, Y