A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain.
A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain.
复制标题
导水管周围灰质 NR2B 含有 NMDA 受体在介导持续性炎性疼痛中的作用
DOI：
10.1186/1744-8069-5-71
复制
发表时间：
2009-12-12
期刊：
影响因子：
3.3
通讯作者：
Zhao MG
中科院分区：
文献类型：
--
作者：
Hu J;Wang Z;Guo YY;Zhang XN;Xu ZH;Liu SB;Guo HJ;Yang Q;Zhang FX;Sun XL;Zhao MG
中脑导水管周围灰质（PAG）是一种在疼痛传递和调节中发挥作用的结构。伤害性刺激增强谷氨酸突触传递并增强PAG中谷氨酸NMDA受体的表达。然而，关于PAG中NMDA受体亚单位在持续性炎性痛过程中的作用却知之甚少。本研究旨在探讨PAG中含有NR 2A和NR 2B的NMDA受体及其对外周痛性炎症的调节作用。通过后爪注射完全弗氏佐剂（CFA）诱导的伤害性刺激引起PAG中含有NR 2B的NMDA受体的上调，而含有NR 2A的NMDA受体没有改变。全细胞膜片钳记录显示，在小鼠炎症痛的慢性阶段，PAG突触中NMDA受体介导的mEPSCs显著增加。PAG局部输注NR 2B拮抗剂Ro 25-6981显著延长大鼠双侧热弧度热刺激的缩足潜伏期。金丝桃苷（Hyperoside，Hyp）是从高山杜鹃（Rhododendron ponticum L.）显著逆转PAG中含有NR 2B的NMDA受体的上调，并对小鼠中的持续性炎症刺激表现出镇痛活性。我们的研究结果提供了强有力的证据表明，含NR 2B的NMDA受体在PAG的上调参与调制外周持续性炎性疼痛。
The midbrain periaqueductal grey (PAG) is a structure known for its roles in pain transmission and modulation. Noxious stimuli potentiate the glutamate synaptic transmission and enhance glutamate NMDA receptor expression in the PAG. However, little is known about roles of NMDA receptor subunits in the PAG in processing the persistent inflammatory pain. The present study was undertaken to investigate NR2A- and NR2B-containing NMDA receptors in the PAG and their modulation to the peripheral painful inflammation. Noxious stimuli induced by hind-paw injection of complete Freund's adjuvant (CFA) caused up-regulation of NR2B-containing NMDA receptors in the PAG, while NR2A-containing NMDA receptors were not altered. Whole-cell patch-clamp recordings revealed that NMDA receptor mediated mEPSCs were increased significantly in the PAG synapse during the chronic phases of inflammatory pain in mice. PAG local infusion of Ro 25-6981, an NR2B antagonist, notably prolonged the paw withdrawal latency to thermal radian heat stimuli bilaterally in rats. Hyperoside (Hyp), one of the flavonoids compound isolated fromRhododendron ponticumL., significantly reversed up-regulation of NR2B-containing NMDA receptors in the PAG and exhibited analgesic activities against persistent inflammatory stimuli in mice. Our findings provide strong evidence that up-regulation of NR2B-containing NMDA receptors in the PAG involves in the modulation to the peripheral persistent inflammatory pain.
登录
查看更多内容
脊髓α-Amino-3-羟基-5-甲基-4-异恶唑丙酸受体受体的作用在完整的弗朗德辅助引起的炎症性疼痛中。
影响因子：
3.3
作者：
Park JS;Yaster M;Guan X;Xu JT;Shih MH;Guan Y;Raja SN;Tao YX
通讯作者：
Tao YX
影响因子：
3
作者：
Cheppudira, Bopaiah Pooviah
通讯作者：
Cheppudira, Bopaiah Pooviah
影响因子：
5.3
作者：
Guo, W;Robbins, MT;Ren, K
通讯作者：
Ren, K
影响因子：
3.6
作者：
Calejesan, AA;Kim, SJ;Zhuo, M
通讯作者：
Zhuo, M
影响因子：
56.9
作者：
Hayashi, Y;Shi, SH;Malinow, R
通讯作者：
Malinow, R