An engineered lantibiotic synthetase that does not require a leader peptide on its substrate.
An engineered lantibiotic synthetase that does not require a leader peptide on its substrate.
复制标题
DOI：
10.1021/ja3017297
复制
发表时间：
2012-04-25
影响因子：
15
通讯作者：
van der Donk, Wilfred A.
中科院分区：
文献类型：
--
作者：
Oman, Trent J.;Knerr, Patrick J.;Bindman, Noah A.;Velasquez, Juan E.;van der Donk, Wilfred A.
核糖体合成和后修饰的肽是一类迅速扩大的天然产物。它们通常通过修饰前体肽（核心肽）的C-末端区段来生物合成。前体肽还含有引导生物合成酶所需的N-末端前导肽。为了生物工程的目的，前导肽是有益的，因为它允许生物合成酶关于核心肽序列的修饰的混杂活性。然而，前导肽也存在缺点，因为它需要存在于核心肽上，然后在随后的步骤中除去。我们表明，融合的前导肽lantibiotic乳酸菌素481的生物合成酶LctM允许蛋白质作用于核心肽没有前导肽。我们说明了使用这种方法制备改进的乳酸菌素481类似物含有非蛋白质氨基酸。
Ribosomally synthesized and post-translationally modified peptides are a rapidly expanding class of natural products. They are typically biosynthesized by modification of a C-terminal segment of the precursor peptide (the core peptide). The precursor peptide also contains an N-terminal leader peptide that is required to guide the biosynthetic enzymes. For bioengineering purposes, the leader peptide is beneficial because it allows promiscuous activity of the biosynthetic enzymes with respect to modification of the core peptide sequence. However, the leader peptide also presents drawbacks as it needs to be present on the core peptide and then removed in a later step. We show that fusing the leader peptide for the lantibiotic lacticin 481 to its biosynthetic enzyme LctM allows the protein to act on core peptides without a leader peptide. We illustrate the use of this methodology for preparation of improved lacticin 481 analogues containing non-proteinogenic amino acids.
登录
查看更多内容
影响因子：
15
作者：
Levengood, Matthew R.;Knerr, Patrick J.;Oman, Trent J.;van der Donk, Wilfred A.
通讯作者：
van der Donk, Wilfred A.
影响因子：
14.8
作者：
Donia, Mohamed S.;Hathaway, Brian J.;Schmidt, Eric W.
通讯作者：
Schmidt, Eric W.
影响因子：
15
作者：
Levengood, Matthew R.;Patton, Gregory C.;van der Donk, Wilfred A.
通讯作者：
van der Donk, Wilfred A.
DOI：
10.1073/pnas.0913677107
发表时间：
2010-06-08
影响因子：
11.1
作者：
Li, Bo;Sher, Daniel;van der Donk, Wilfred A.
通讯作者：
van der Donk, Wilfred A.
影响因子：
4.8
作者：
Lubelski, Jacek;Khusainov, Rustem;Kuipers, Oscar P.
通讯作者：
Kuipers, Oscar P.