Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma: unique viral latency in the tumour cells.
Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma: unique viral latency in the tumour cells.
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DOI：
10.1038/bjc.1996.412
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发表时间：
1996-08
影响因子：
8.8
通讯作者：
Osato, T
中科院分区：
文献类型：
--
作者：
Sugiura, M;Imai, S;Tokunaga, M;Koizumi, S;Uchizawa, M;Okamoto, K;Osato, T
关键词：
尽管最近不断积累关于 Epstein-Barr 病毒 (EBV) 与胃癌关联的病例证据，但 EBV 与胃上皮细胞之间的相互作用在很大程度上尚不清楚。在这项研究中，我们检测了七种 EBV 阳性胃癌组织的 mRNA 水平上的病毒基因表达，这将有助于对人上皮细胞中 EBV 致癌性的研究。逆转录PCR分析显示，所有7种EBV阳性肿瘤组织均持续表达EBV核抗原（EBNA）1 mRNA，但不表达EBNA2 mRNA。 EBNA 转录从三个 EBNA 启动子 Qp 之一启动：相比之下，Cp 和 Wp 都是沉默的，从而导致 EBNA2 mRNA 的缺失。 7 例中有 3 例检测到潜伏膜蛋白 (LMP) 2A mRNA；然而，在任何测试的肿瘤中均未检测到 LMP1 和 LMP2B mRNA。在所有病例中都可以检测到病毒基因组 BamHI-A 区域的转录本。 BZLF1 mRNA 及其产物（EBV 复制的早期基因）在其中任何一个中均不表达，从而表明肿瘤细胞以紧密潜伏的形式携带 EBV 基因组。这些发现进一步扩展了我们之前关于胃癌细胞中EBV潜伏期在蛋白质水平上的数据，并证实肿瘤中病毒基因表达的程序更类似于以伯基特淋巴瘤为代表的“潜伏期I”，而不是大多数鼻咽癌所代表的“潜伏期II”。
Although case-oriented evidence for an association of Epstein-Barr virus (EBV) with gastric carcinoma has been accumulating recently, the interaction(s) between EBV and gastric epithelial cells is/are largely unknown. In this study, we examined seven EBV-positive gastric carcinoma tissues for viral gene expression at the mRNA level, from which studies on the EBV oncogenicity in human epithelial cells will benefit. Reverse transcription-PCR analysis showed that all seven EBV-positive tumour tissues constitutively expressed EBV nuclear antigen (EBNA) 1 mRNA, but not EBNA2 mRNA. The EBNA transcription was initiated from one of three EBNA promoters, Qp: by contrast, both Cp and Wp were silent, thus resulting in the lack of EBNA2 mRNA. Latent membrane protein (LMP) 2A mRNA was detected in three of seven cases; however, neither LMP1 nor LMP2B mRNA was detected in any of the tumours tested. Transcripts from the BamHI-A region of the viral genome were detectable in all cases. BZLF1 mRNA and the product, an immediate-early gene for EBV replication, was not expressed in any of them, thereby suggesting that the tumour cells carried EBV genomes in a tightly latent form. These findings further extended our previous data regarding EBV latency in gastric carcinoma cells at the protein level, and have affirmed that the programme of viral gene expression in the tumour more closely resembles 'latency I' represented by Burkitt's lymphoma than 'latency II' represented by the majority of nasopharyngeal carcinomas.
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影响因子：
64.8
作者：
LI, QX;YOUNG, LS;RICKINSON, AB
通讯作者：
RICKINSON, AB
DOI：
10.1084/jem.176.1.157
发表时间：
1992-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Murray RJ;Kurilla MG;Brooks JM;Thomas WA;Rowe M;Kieff E;Rickinson AB
通讯作者：
Rickinson AB
影响因子：
64.8
作者：
DAWSON, CW;RICKINSON, AB;YOUNG, LS
通讯作者：
YOUNG, LS
影响因子：
20.3
作者：
KORBJUHN, P;ANAGNOSTOPOULOS, I;STEIN, H
通讯作者：
STEIN, H
DOI：
10.1073/pnas.71.8.3283
发表时间：
1974-01-01
影响因子：
11.1
作者：
KLEIN, G;LINDAHL, T;SUNDSTROM, C
通讯作者：
SUNDSTROM, C