Synaptic physiology in the cochlear nucleus angularis of the chick.
Synaptic physiology in the cochlear nucleus angularis of the chick.
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雏鸡耳蜗角核的突触生理学。
DOI：
10.1152/jn.00898.2004
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发表时间：
2005
影响因子：
2.5
通讯作者：
Carr,CatherineE
中科院分区：
文献类型：
--
作者：
MacLeod,KatrinaM;Carr,CatherineE
角核 (NA) 是鸟类的两个耳蜗核之一，对于处理声音强度以进行定位非常重要，并且很可能在声音识别和其他听觉任务中发挥作用。由于耳蜗核的听觉神经输入的突触特性是听觉信息转换的基础，因此我们使用胚胎鸡听觉脑干切片（E16-E20）的全细胞膜片钳记录，研究了这些突触到 NA 神经元的特性。我们测量了自发兴奋性突触后电流 (EPSC)，并通过使用听神经的细胞外刺激诱发 EPSC 和兴奋性突触后电位 (EPSP)。这些兴奋性 EPSC 由 AMPA 和 N-甲基-d-天冬氨酸 (NMDA) 受体介导。由 AMPA 受体介导的自发 EPSC 具有亚毫秒衰减动力学（E19 为 556 μs），与其他听觉脑干区域的衰减动力学相当。随着发育年龄的增长，自发性 EPSC 的幅度增加并变得更快。诱发 EPSC 和 EPSP 幅度按刺激强度分级。最小刺激引起的 EPSC 平均振幅是平均自发 EPSC 振幅的两倍（~110 vs.~55 pA），表明单纤维与每个突触后 NA 神经元进行多次接触。由于其尺寸较小，最小 EPSP 低于阈值，我们估计至少需要三到五个输入才能达到阈值。与快速 EPSC 相比，NA 中的 EPSP 的衰减时间常数约为 12.5 ms，这很大程度上受到膜时间常数的影响。因此，NA 神经元在空间和时间上整合来自多个听觉神经传入的听觉信息。
Nucleus angularis (NA), one of the two cochlear nuclei in birds, is important for processing sound intensity for localization and most likely has role in sound recognition and other auditory tasks. Because the synaptic properties of auditory nerve inputs to the cochlear nuclei are fundamental to the transformation of auditory information, we studied the properties of these synapses onto NA neurons using whole cell patch-clamp recordings from auditory brain stem slices from embryonic chickens (E16–E20). We measured spontaneous excitatory postsynaptic currents (EPSCs), and evoked EPSCs and excitatory postsynaptic potentials (EPSPs) by using extracellular stimulation of the auditory nerve. These excitatory EPSCs were mediated by AMPA andN-methyl-d-aspartate (NMDA) receptors. The spontaneous EPSCs mediated by AMPA receptors had submillisecond decay kinetics (556 μs at E19), comparable with those of other auditory brain stem areas. The spontaneous EPSCs increased in amplitude and became faster with developmental age. Evoked EPSC and EPSP amplitudes were graded with stimulus intensity. The average amplitude of the EPSC evoked by minimal stimulation was twice as large as the average spontaneous EPSC amplitude (∼110 vs. ∼55 pA), suggesting that single fibers make multiple contacts onto each postsynaptic NA neuron. Because of their small size, minimal EPSPs were subthreshold, and we estimate at least three to five inputs were required to reach threshold. In contrast to the fast EPSCs, EPSPs in NA had a decay time constant of ∼12.5 ms, which was heavily influenced by the membrane time constant. Thus NA neurons spatially and temporally integrate auditory information arriving from multiple auditory nerve afferents.
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鸟类大细胞核兴奋性突触传递的电压钳分析。
DOI：
10.1113/jphysiol.1994.sp020346
发表时间：
1994
期刊：
The Journal of physiology
影响因子：
--
作者：
Zhang,S;Trussell,LO
通讯作者：
Trussell,LO
影响因子：
2.5
作者：
Soares,Daphne;Chitwood,RaymondA;Hyson,RichardL;Carr,CatherineE
通讯作者：
Carr,CatherineE
猫头鹰的耳蜗核处理不同的声音定位线索。
DOI：
10.1121/1.392499
发表时间：
1985
期刊：
The Journal of the Acoustical Society of America
影响因子：
--
作者：
Konishi,M;Sullivan,WE;Takahashi,T
通讯作者：
Takahashi,T
雏鸡耳蜗神经中声音强度的编码。
影响因子：
2.5
作者：
J. Saunders;C. Ventetuolo;S. Plontke;B. Weiss
通讯作者：
B. Weiss
影响因子：
5.5
作者：
BARNESDAVIES, M;FORSYTHE, ID
通讯作者：
FORSYTHE, ID