Cellular traction stresses mediate extracellular matrix degradation by invadopodia.
Cellular traction stresses mediate extracellular matrix degradation by invadopodia.
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DOI：
10.1016/j.actbio.2013.12.058
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发表时间：
2014-05
影响因子：
9.7
通讯作者：
Parekh, Aron
中科院分区：
文献类型：
--
作者：
Jerrell, Rachel J.;Parekh, Aron
在肿瘤发生过程中，基质刚性可以通过改变细胞的增殖和迁移来驱动致癌转化。细胞通过肌动球蛋白收缩产生的细胞内张力力感知细胞外基质(ECM)的机械特性。这些收缩力作为牵引力传递到基质表面，从而调节与ECM的机械相互作用。基质刚性已被证明可以通过细胞骨架结构增加蛋白水解性ECM的降解，这种结构对癌症的进展至关重要，这表明细胞的收缩促进了侵袭行为。然而，牵引应力的增加和减少都与转移行为有关。因此，细胞收缩在侵袭性迁移导致转移中的作用尚不清楚。为了确定细胞牵拉应力和内侧足细胞活性之间的关系，我们用不同硬度的聚丙烯酰胺凝胶表征了侵袭性癌细胞系的侵袭和收缩特性。我们发现ECM降解和拉伸应力是基质刚性的线性函数。使用花盏花素A来增强肌球蛋白的收缩能力，我们还发现牵引应力对细胞外基质的降解有很强的预测作用。总体而言，我们的数据表明，细胞力的产生可能在侵袭和转移中发挥重要作用，因为它介导了对肿瘤微环境的机械特性做出反应的内向活动。
During tumorigenesis, matrix rigidity can drive oncogenic transformation via altered cellular proliferation and migration. Cells sense extracellular matrix (ECM) mechanical properties with intracellular tensile forces generated by actomyosin contractility. These contractile forces are transmitted to the matrix surface as traction stresses which mediate mechanical interactions with the ECM. Matrix rigidity has been shown to increase proteolytic ECM degradation by cytoskeletal structures known as invadopodia that are critical for cancer progression suggesting that cellular contractility promotes invasive behavior. However, both increases and decreases in traction stresses have been associated with metastatic behavior. Therefore, the role of cellular contractility in invasive migration leading to metastasis is unclear. To determine the relationship between cellular traction stresses and invadopodia activity, we characterized the invasive and contractile properties of an aggressive carcinoma cell line utilizing polyacrylamide gels of different rigidities. We found that ECM degradation and traction stresses were linear functions of matrix rigidity. Using calyculin A to augment myosin contractility, we also found that traction stresses were strongly predictive of ECM degradation. Overall, our data suggest that cellular force generation may play an important part in invasion and metastasis by mediating invadopodia activity in response to the mechanical properties of the tumor microenvironment.
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影响因子：
9.2
作者：
Collin, Olivier;Na, Sungsoo;Chowdhury, Farhan;Hong, Michael;Shin, Myung Eun;Wang, Fei;Wang, Ning
通讯作者：
Wang, Ning
影响因子：
3.3
作者：
Beaty BT;Sharma VP;Bravo-Cordero JJ;Simpson MA;Eddy RJ;Koleske AJ;Condeelis J
通讯作者：
Condeelis J
影响因子：
4
作者：
Collin, Olivier;Tracqui, Philippe;Planus, Emmanuelle
通讯作者：
Planus, Emmanuelle
DOI：
10.1597/08-012.1
发表时间：
2009-05-01
期刊：
CLEFT PALATE-CRANIOFACIAL JOURNAL
影响因子：
--
作者：
Birch, M. J.;Srodon, P. D.
通讯作者：
Srodon, P. D.
影响因子：
2.5
作者：
BECKETT, MA;WEICHSELBAUM, RR
通讯作者：
WEICHSELBAUM, RR