RBOH1-dependent H2O2 production and subsequent activation of MPK1/2 play an important role in acclimation-induced cross-tolerance in tomato.
RBOH1-dependent H2O2 production and subsequent activation of MPK1/2 play an important role in acclimation-induced cross-tolerance in tomato.
复制标题
RBOH1 依赖的 H2O2 产生和随后的 MPK1/2 激活在番茄驯化诱导的交叉耐受性中发挥重要作用
DOI：
10.1093/jxb/ert404
复制
发表时间：
2014-02
影响因子：
6.9
通讯作者：
Yu JQ
中科院分区：
文献类型：
--
作者：
Zhou J;Xia XJ;Zhou YH;Shi K;Chen Z;Yu JQ
H_2O_2和丝裂原活化蛋白激酶（MAPK）级联反应在植物逆境响应中发挥重要作用，但它们在植物适应反应中的作用尚不清楚。本研究探讨了H2 O2和MPK 1/2在驯化诱导的番茄交叉耐性中的作用。温和的寒冷，百草枯，干旱作为驯化刺激增强耐受性更严重的随后的寒冷，光氧化和干旱胁迫。驯化诱导的交叉耐受性与番茄中RBOH 1和胁迫和防御相关基因的转录水平增加、质外体H2 O2积累升高、NADPH氧化酶和抗氧化酶活性增加、谷胱甘肽氧化还原状态降低和MPK 1/2活化有关。病毒诱导的RBOH 1、MPK 1和MPK 2或MPK 1/2基因沉默都损害了驯化诱导的交叉耐受性和相关的应激反应。两者合计，这些结果强烈表明，驯化诱导的交叉耐受性在很大程度上归因于RBOH 1依赖的过氧化氢的生产在质外体，这可能随后激活MPK 1/2诱导应激反应。
H2O2 and mitogen-activated protein kinase (MAPK) cascades play important functions in plant stress responses, but their roles in acclimation response remain unclear. This study examined the functions of H2O2 and MPK1/2 in acclimation-induced cross-tolerance in tomato plants. Mild cold, paraquat, and drought as acclimation stimuli enhanced tolerance to more severe subsequent chilling, photooxidative, and drought stresses. Acclimation-induced cross-tolerance was associated with increased transcript levels of RBOH1 and stress- and defence-related genes, elevated apoplastic H2O2 accumulation, increased activity of NADPH oxidase and antioxidant enzymes, reduced glutathione redox state, and activation of MPK1/2 in tomato. Virus-induced gene silencing of RBOH1, MPK1, and MPK2 or MPK1/2 all compromised acclimation-induced cross-tolerance and associated stress responses. Taken together, these results strongly suggest that acclimation-induced cross-tolerance is largely attributed to RBOH1-dependent H2O2 production at the apoplast, which may subsequently activate MPK1/2 to induce stress responses.
登录
查看更多内容
脱落酸信号传导中丝裂原激活蛋白激酶级联对玉米 NADPH 氧化酶的正反馈调节
影响因子：
6.9
作者：
Lin F;Ding H;Wang J;Zhang H;Zhang A;Zhang Y;Tan M;Dong W;Jiang M
通讯作者：
Jiang M
影响因子：
7.2
作者：
Dat, JF;Pellinen, R;Van Breusegem, F
通讯作者：
Van Breusegem, F
DOI：
10.1098/rstb.2002.1141
发表时间：
2002-10-29
影响因子：
6.3
作者：
Baena-González, E;Aro, EM
通讯作者：
Aro, EM
影响因子：
11.6
作者：
Bestwick, CS;Brown, IR;Mansfield, JW
通讯作者：
Mansfield, JW
一氧化氮在黄瓜油菜素类固醇过氧化氢依赖性诱导非生物胁迫耐受性中的作用
影响因子：
7.3
作者：
Cui, Jin-Xia;Zhou, Yan-Hong;Yu, Jing-Quan
通讯作者：
Yu, Jing-Quan