Transcriptional regulatory regions of gap43 needed in developing and regenerating retinal ganglion cells.
Transcriptional regulatory regions of gap43 needed in developing and regenerating retinal ganglion cells.
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DOI：
10.1002/dvdy.22190
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发表时间：
2010-02
影响因子：
2.5
通讯作者：
Udvadia, Ava J.
中科院分区：
文献类型：
--
作者：
Kusik, Brandon W.;Hammond, Dena R.;Udvadia, Ava J.
哺乳动物和鱼类在表达轴突生长相关基因以响应CNS损伤的能力上不同，这导致了它们在CNS再生能力上的差异。以前，我们证明了轴突生长相关基因，gap43，大鼠启动子的区域，足以促进报告基因在发育中的斑马鱼神经系统的表达是不足以促进表达再生视网膜神经节细胞在斑马鱼。最近，我们确定了一个3.6 kb的gap43启动子片段从河豚，红鳍东方鲀（河豚），可以促进报告基因的表达在发育和再生。使用启动子缺失分析，我们已经发现了3.6 kb的河豚gap43启动子的区域是必要的再生，但不发展视网膜神经节细胞的表达。在3.6 kb启动子中，我们已经鉴定出在鱼类中高度保守的元件，以及在鱼类、哺乳动物和鸟类中保守的元件。
Mammals and fish differ in their ability to express axon growth-associated genes in response to CNS injury, which contributes to the differences in their ability for CNS regeneration. Previously we demonstrated that for the axon growth-associated gene, gap43, regions of the rat promoter that are sufficient to promote reporter gene expression in the developing zebrafish nervous system are not sufficient to promote expression in regenerating retinal ganglion cells in zebrafish. Recently we identified a 3.6 kb gap43 promoter fragment from the pufferfish, Takifugu rubripes (fugu), that can promote reporter gene expression during both development and regeneration. Using promoter deletion analysis, we have found regions of the 3.6 kb fugu gap43 promoter that are necessary for expression in regenerating, but not developing retinal ganglion cells. Within the 3.6 kb promoter we have identified elements that are highly conserved among fish, as well as elements conserved among fish, mammals, and birds.
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