MMP-9-Related microRNAs as Prognostic Markers for Hemorrhagic Transformation in Cardioembolic Stroke Patients
MMP-9-Related microRNAs as Prognostic Markers for Hemorrhagic Transformation in Cardioembolic Stroke Patients
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MMP-9 相关 microRNA 作为心源性卒中患者出血性转化的预后标志物
DOI：
10.3389/fneur.2019.00945
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发表时间：
2019-09
影响因子：
3.4
通讯作者：
Wang Deren
中科院分区：
文献类型：
--
作者：
Zheng Lukai;Xiong Yao;Liu Junfeng;Yang Xue;Wang Lu;Zhang Shuting;Liu Ming;Wang Deren
研究表明，调节基质金属蛋白酶（MMP）-9表达的microRNA可能参与心源性栓塞性卒中后出血性转化（HT），因此我们研究了此类microRNA是否可以预测急性心源性栓塞性卒中患者的HT。从后来经历HT（n = 29）或没有经历HT（n = 29）的患者中前瞻性地收集血液样品，并且基于三个microRNA数据库分析样品中鉴定为与MMP-9相关的八种microRNA。通过定量实时聚合酶链反应（qRT-PCR）分析了58名患者中28名患者的这些microRNA的表达水平，其中14名患有HT，14名没有。鉴定出四种差异表达的miRNA：hsa-miR-21- 5 p、hsa-miR-206、hsa-miR-491- 5 p和hsa-miR-3123。随后对所有58名患者的这四种miRNA进行qRT-PCR分析，结果显示，HT患者中miR-21- 5 p、miR-206和miR-3123的水平显著高于非HT患者，而两组之间miR-491- 5 p的表达相似。用于预测HT的接受者工作特征曲线下面积对于miR-21- 5 p为0.677（95%CI 0.535-0.818），对于miR-206为0.687（95%CI 0.543-0.830），对于miR-3123为0.661（95%CI 0.512-0.810）。我们的研究结果表明，这三种microRNA可能是心源性栓塞性卒中后HT的预后标志物，这一点应通过未来的大样本研究进行验证。
Studies suggest that microRNAs that regulate expression of matrix metalloproteinase (MMP)-9 may be involved in hemorrhagic transformation (HT) after cardioembolic stroke, so we examined whether such microRNAs could predict HT in acute cardioembolic stroke patients. Blood samples were prospectively collected from patients who later experienced HT (n = 29) or did not (n = 29), and the samples were assayed for eight microRNAs identified as related to MMP-9 based on three microRNA databases. Expression levels of these microRNAs were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) in 28 of the 58 patients, 14 of whom suffered HT and 14 of whom did not. Four differentially expressed miRNAs were identified: hsa-miR-21-5p, hsa-miR-206, hsa-miR-491-5p, and hsa-miR-3123. Subsequent qRT-PCR analysis of these four miRNAs across all 58 patients showed that levels of miR-21-5p, miR-206, and miR-3123 were significantly higher in patients with HT than in those without HT, while expression of miR-491-5p was similar between the two groups. The area under the receiver operating characteristic curve for predicting HT was 0.677 (95% CI 0.535–0.818) for miR-21-5p, 0.687 (95% CI 0.543–0.830) for miR-206, and 0.661 (95% CI 0.512–0.810) for miR-3123. Our results suggest that these three microRNAs may be prognostic markers for HT after cardioembolic stroke, which should be verified by future studies with large samples.
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DOI：
10.3760/cma.j.issn.0254-6450.2012.11.018
发表时间：
2012-11
影响因子：
--
作者：
Jiajun Chen;Shuang Zhang;Yunhua Zhao;X. Jin;Yuan-bing Li;Li-hong Huang
通讯作者：
Jiajun Chen;Shuang Zhang;Yunhua Zhao;X. Jin;Yuan-bing Li;Li-hong Huang
影响因子：
8.3
作者：
A-ChingChao;Hung-YiHsu;Chih-PingChung;Chung-HsiangLiu;Chih-HungChen;Michael Mu-HuoTeng;Giia-SheunPeng;Wen-YungSheng;Han HwaHu
通讯作者：
A-ChingChao;Hung-YiHsu;Chih-PingChung;Chung-HsiangLiu;Chih-HungChen;Michael Mu-HuoTeng;Giia-SheunPeng;Wen-YungSheng;Han HwaHu
影响因子：
4.3
作者：
Darabi, Faramarz;Aghaei, Mahmoud;Sarrafzadegan, Nizal
通讯作者：
Sarrafzadegan, Nizal
影响因子：
8.3
作者：
M. Mulder;Hester F. Lingsma;D. Dippel
通讯作者：
M. Mulder;Hester F. Lingsma;D. Dippel
影响因子：
8.3
作者：
Jauch, Edward C.;Saver, Jeffrey L.;Yonas, Howard
通讯作者：
Yonas, Howard