Endothelial Plaques as Sign of Hyphae Infiltration of Descemet's Membrane in Fungal Keratitis
Endothelial Plaques as Sign of Hyphae Infiltration of Descemet's Membrane in Fungal Keratitis
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内皮斑块是真菌性角膜炎后弹力层菌丝浸润的标志
DOI：
10.1155/2020/6083854
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发表时间：
2020-05
影响因子：
1.9
通讯作者：
Gao Hua
中科院分区：
文献类型：
--
作者：
Qi Xiaolin;Liu Ting;Du Man;Gao Hua
背景探讨真菌性角膜炎患者角膜内皮斑块与真菌菌丝浸润的关系。方法对2013年1月至2017年3月间行角膜移植术的60例真菌性角膜炎患者进行回顾性横断面研究。内皮斑块分级如下：1级、1 - 3级内皮斑块；2级，4-8级内皮斑块；3级，超过8个内皮斑块或致密合并的内皮斑块。对真菌病原菌培养及病膜组织病理学进行了评价。结果根据内皮斑块分级，1级3例，2级29例，3级28例。57例2级和3级内皮斑块患者行PK手术，3例1级内皮斑块患者行DALK手术。病原菌以曲霉属为主（63.2%）。所有57例2级和3级患者的Descemet膜均有真菌菌丝，这是基于钙荧光白染色或PAS染色。在3级患者中，在Descemet的膜中发现更多的菌丝和炎症细胞。内皮斑块免疫组化染色显示大多数细胞CD15和CD68阳性。在随访期间，3例接受DALK的患者中有2例复发性真菌性角膜炎。结论内皮斑块被认为是菌丝浸润Descemet膜的标志。术中一旦内皮中发现斑块，应进行PK。
Background To evaluate the relationship between corneal endothelial plaques and fungal hyphae infiltration in fungal keratitis. Methods Retrospective cross-sectional study of 60 fungal keratitis patients who underwent keratoplasty between January 2013 and March 2017. The endothelial plaques were graded as follows: grade 1, 1–3 endothelial plaques; grade 2, 4–8 endothelial plaques; and grade 3, more than 8 endothelial plaques or dense, merging endothelial plaques. The fungal pathogen culture and histopathology of diseased Descemet's membrane were evaluated. Results According to endothelial plaque grading, 3 patients were grade 1, 29 patients were grade 2, and 28 patients were grade 3. The PK surgery was performed in 57 patients with endothelial plaques of grade 2 and grade 3 and DALK surgery in 3 patients of grade 1. The predominating fungal pathogens were Aspergillus species (63.2%). All 57 patients with grade 2 and grade 3 had fungal hyphae in Descemet's membrane based on calcofluor white staining or PAS staining. In patients with grade 3, more hyphae and inflammatory cells were found in Descemet's membrane. The immunohistochemical staining of endothelial plaques revealed that CD15 and CD68 were positive in most cells. During the follow-up, 2 out of 3 patients who underwent DALK had recurrent fungal keratitis. Conclusions Endothelial plaques are considered as a sign of hyphae infiltrating Descemet's membrane. PK should be performed once plaques are detected in endothelium during the surgery.
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影响因子：
4.2
作者：
Ti, Seng-El;Scott, Angus;Tan, Donald T. H.
通讯作者：
Tan, Donald T. H.
DOI：
10.1080/02681219180000631
发表时间：
1991-11
期刊：
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
影响因子：
--
作者：
H. Kiryu;S. Yoshida;Y. Suenaga;M. Asahi
通讯作者：
H. Kiryu;S. Yoshida;Y. Suenaga;M. Asahi
与巨细胞病毒内皮炎相关的免疫环形成。
影响因子：
4.2
作者：
Chun;Y. Tsai
通讯作者：
Y. Tsai
角膜移植后复发性真菌性角膜炎的危险因素、临床特征和结果
影响因子：
13.7
作者：
Shi, Weiyun;Wang, Ting;Li, Huiping
通讯作者：
Li, Huiping
影响因子：
2
作者：
Dong, XG;Shi, WY;Xie, LX
通讯作者：
Xie, LX