Structural variability and differentiation of niches in the rhizosphere and endosphere bacterial microbiome of moso bamboo (Phyllostachys edulis).
Structural variability and differentiation of niches in the rhizosphere and endosphere bacterial microbiome of moso bamboo (Phyllostachys edulis).
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毛竹根际和内圈细菌微生物群的结构变异和生态位分化
DOI：
10.1038/s41598-021-80971-9
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发表时间：
2021-01-15
影响因子：
4.6
通讯作者：
Pan H
中科院分区：
文献类型：
--
作者：
Yuan ZS;Liu F;Liu ZY;Huang QL;Zhang GF;Pan H
植物微生物群在植物生产力、营养吸收、抗逆性和开花中起着关键作用。毛竹的开花一直是研究的热点。开花机理与养分吸收、温度、激素平衡及关键基因调控有关。然而，毛竹微生物群与其开花之间的联系尚不清楚。本研究采集了毛竹开花期和非开花期的根际土壤、根茎、根和叶样品，利用16 S rRNA扩增子Illumina测序技术对毛竹不同开花期的细菌群落进行了分离。共鉴定出5442个OTU，其中根际土壤OTU数量远高于其他样品。主成分分析（PCA）和系统聚类分析（Bray Curtis dis）结果表明，毛竹根际土壤和内生组织中的细菌微生物与非根际土壤、根际细菌和内围微生物存在显著差异。此外，根和根际土壤的主成分分析显示，不同的微生物群落结构之间的开花和非开花竹。通过对核心微生物的分析，发现黄杆菌、芽孢杆菌和寡养单胞菌在毛竹对N元素的吸收中起着重要作用，可能影响毛竹的花期。我们的研究结果描绘了这种植物复杂的宿主-微生物相互作用。探讨了细菌微生物群对毛竹开花的潜在影响，为毛竹的开发利用提供依据。
The plant microbiota play a key role in plant productivity, nutrient uptake, resistance to stress and flowering. The flowering of moso bamboo has been a focus of study. The mechanism of flowering is related to nutrient uptake, temperature, hormone balance and regulation of key genes. However, the connection between microbiota of moso bamboo and its flowering is unknown. In this study, samples of rhizosphere soil, rhizomes, roots and leaves of flowering and nonflowering plants were collected, and 16S rRNA amplicon Illumina sequencing was utilized to separate the bacterial communities associated with different flowering stages of moso bamboo. We identified 5442 OTUs, and the number of rhizosphere soil OTUs was much higher than those of other samples. Principal component analysis (PCA) and hierarchical clustering (Bray Curtis dis) analysis revealed that the bacterial microorganisms related to rhizosphere soil and endophytic tissues of moso bamboo differed significantly from those in bulk soil and rhizobacterial and endosphere microbiomes. In addition, the PCA analyses of root and rhizosphere soil revealed different structures of microbial communities between bamboo that is flowering and not flowering. Through the analysis of core microorganisms, it was found thatFlavobacterium,BacillusandStenotrophomonasplayed an important role in the absorption of N elements, which may affect the flowering time of moso bamboo. Our results delineate the complex host-microbe interactions of this plant. We also discuss the potential influence of bacterial microbiome in flowering, which can provide a basis for the development and utilization of moso bamboo.
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影响因子：
5.6
作者：
Lebeis SL
通讯作者：
Lebeis SL
DOI：
10.2307/3668597
发表时间：
1999-01-01
期刊：
Systematics and Geography of Plants
影响因子：
--
作者：
Gielis, J.;Goetghebeur, P.;Debergh, P.
通讯作者：
Debergh, P.
影响因子：
4.4
作者：
Gottel, Neil R.;Castro, Hector F.;Schadt, Christopher W.
通讯作者：
Schadt, Christopher W.
影响因子：
4.9
作者：
Isagi, Y;Shimada, K;Watanabe, S
通讯作者：
Watanabe, S
影响因子：
4.4
作者：
Krzmarzick, Mark J.;Crary, Benjamin B.;Novak, Paige J.
通讯作者：
Novak, Paige J.