Neuromuscular adaptability of male and female rats to muscle unloading.
Neuromuscular adaptability of male and female rats to muscle unloading.
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DOI：
10.1002/jnr.24129
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发表时间：
2018-03
影响因子：
4.2
通讯作者：
Schaffrey EC
中科院分区：
文献类型：
--
作者：
Deschenes MR;Adan MA;Kapral MC;Kressin KA;Leathrum CM;Seo A;Li S;Schaffrey EC
此前，已有研究表明，在肌肉卸载后，男性和女性在神经肌肉功能方面经历了不同的适应不良。作为后续研究，本研究试图确定男性和女性神经肌肉系统对肌肉卸载是否表现出相似或不同的形态适应。20只年轻成年雄性大鼠和20只年轻成年雌性大鼠被随机分配到两种治疗方案中的一种：肌肉卸载或控制条件。干预2周后，应用免疫荧光技术对神经肌肉接头(NMJ)突触前和突触后的特征进行量化，并评估比目鱼肌、足底肌和内侧副韧带肌的肌纤维形态(大小和纤维类型组成)。然后使用对性别和治疗有主效应的双因素方差分析来确定结构参数之间的统计学差异(P≤0.05)。对NMJ的分析表明，男性和女性之间一直缺乏差异。总体而言，NMJ也被发现对卸货的影响具有抵抗力。然而，当检查肌纤维分布时，在所检查的每一块肌肉中，男性的肌纤维明显大于女性的。仅在主要负重的比目鱼肌中，卸载才导致显著的肌纤维萎缩。只有EDL在卸载诱导的肌纤维类型分布(类型II→I型)上表现出差异。这些数据表明，神经肌肉系统的不同组成部分(NMJ，肌纤维)对卸载的反应是独特的，性别影响肌纤维类型，但不影响NMJ。此外，似乎只有那些习惯活动模式因卸货而受到干扰的肌肉，即比目鱼肌，才能适应这种干预。最上面一排的图像显示，男性和女性神经肌肉接头的形态没有区别。相反，下面所示的肌纤维表明男性的肌纤维比女性的大(红色=I型，蓝色=IIA型，未染色=IIX型，绿色=IIB型)。
Previously, it has been shown that following muscle unloading, males and females experience different maladaptations in neuromuscular function. As a follow-up, the present investigation sought to determine if male and female neuromuscular systems demonstrated similar, or disparate morphological adaptations to muscle unloading. Twenty young adult male, and 20 young adult female rats were randomly assigned to one of two treatment protocols: muscle unloading, or control conditions. Following the 2 week intervention period, immunofluorescent procedures were used to quantify pre- and post-synaptic features of neuromuscular junctions (NMJs), and to assess myofiber profiles (size and fiber type composition) of the soleus, plantaris, and EDL muscles. A 2-way ANOVA with main effects for sex and treatment was then used to identify statistically significant (P ≤ 0.05) differences among structural parameters. Analysis of NMJs showed a consistent lack of differences between males and females. Overall, NMJs were also found to be resistant to the effects of unloading. When examining myofiber profiles, however, male myofibers were revealed to be significantly larger than female ones in each of the muscles examined. Unloading resulted in significant myofiber atrophy only in the primarily weight-bearing soleus muscle. Only the EDL showed unloading-induced differences in myofiber type distribution (Type II → I). These data indicate that different components of the neuromuscular system (NMJs, myofibers) respond uniquely to unloading, and that sex affects myofiber type profiles, but not NMJs. Moreover, it appears that only muscles that have their habitual activity patterns disturbed by unloading, i.e., the soleus, adapt to that intervention. Images in the top row show that the morphology of male and female neuromuscular junctions do not differ. Conversely, myofibers shown below demonstrate that male myofibers are larger than female ones (red = Type I, blue = Type IIA, unstained = Type IIX, and green = Type IIB fibers).
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影响因子：
4
作者：
Fox, J;Garber, P;Thompson, LV
通讯作者：
Thompson, LV
影响因子：
5.5
作者：
Hortobágyi, T;Dempsey, L;Dohm, L
通讯作者：
Dohm, L
DOI：
10.1002/neu.10271
发表时间：
2003-12-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Deschenes, MR;Wilson, MH
通讯作者：
Wilson, MH
影响因子：
3.3
作者：
ARMSTRONG, RB;LAUGHLIN, MH
通讯作者：
LAUGHLIN, MH
影响因子：
3.3
作者：
Delp, MD;Duan, CP
通讯作者：
Duan, CP