Intravital imaging of metastatic behavior through a mammary imaging window.

Intravital imaging of metastatic behavior through a mammary imaging window.
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DOI:
10.1038/nmeth.1269
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发表时间:
2008-12
期刊:
影响因子:
48
通讯作者:
van Rheenen, Jacco
van Rheenen, Jacco
中科院分区:
生物学1区
文献类型:
--
作者:
Kedrin, Dmitriy;Gligorijevic, Bojana;Wyckoff, Jeffrey;Verkhusha, Vladislav V.;Condeelis, John;Segall, Jeffrey E.;van Rheenen, Jacco

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我们报告了一种技术,以评估相同的肿瘤微环境在活体小鼠多个活体成像会话。光学标记原位乳腺癌中表达光可切换蛋白的个体肿瘤细胞,并通过乳腺成像窗(MIW)跟踪延长的时间。我们首次证明,同一原位乳腺肿瘤中的两种不同微环境对肿瘤细胞的侵袭和内渗产生不同的影响。
We report a technique to evaluate the same tumor microenvironment over multiple intravital imaging sessions in living mice. Individual tumor cells expressing photoswitchable proteins in an orthotopic mammary carcinoma are optically marked and followed for extended times through a Mammary Imaging Window (MIW). We demonstrate, for the first time, that two distinct microenvironments in the same orthotopic mammary tumor affect differently the invasion and intravasation of tumor cells.
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