Human CD1 dimeric proteins as indispensable tools for research on CD1-binding lipids and CD1-restricted T cells.
Human CD1 dimeric proteins as indispensable tools for research on CD1-binding lipids and CD1-restricted T cells.
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DOI：
10.1016/j.jim.2009.04.002
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发表时间：
2009-06-30
影响因子：
2.2
通讯作者：
Tsuji, Moriya
中科院分区：
文献类型：
--
作者：
Shiratsuchi, Takayuki;Schneck, Jonathan;Kawamura, Akira;Tsuji, Moriya
抗原呈递分子通过引发和激活T细胞在先天性和过继性免疫应答中起重要作用。其中，CD 1分子已被鉴定为向CD 1限制性T细胞呈递外源性和内源性脂质抗原。然而，CD1限制性T细胞参与自身免疫性疾病和防御感染性疾病仍然是未知的。识别与CD1分子结合的新型抗原脂质并了解CD1限制性T细胞的作用应该会导致疫苗的成功开发，因为脂质可以用作抗原，也可以用作佐剂。在本文中，我们已经构建了功能性重组人CD 1二聚体蛋白，并建立了竞争性ELISA法来测量脂质结合的CD 1分子使用的CD 1二聚体。通过使用竞争性ELISA测定，我们能够证明鼠疟原虫的脂质提取物实际上可以加载到CD 1分子上。此外，我们已经证明，人工抗原呈递细胞，其中包括与CD1d二聚体和抗CD28抗体包被的磁珠，刺激和扩增的人不变的NKT细胞作为自体未成熟的DC有效。在目前的研究中提出的一套工具应该是有价值的筛选各种CD1分子结合的脂质抗原和分离CD1限制性T细胞。
Antigen presenting molecules play an important role in both innate and adoptive immune responses by priming and activating T cells. Among them, CD1 molecules have been identified to present both exogenous and endogenous lipid antigens to CD1-restricted T cells. The involvement of CD1-restricted T cells in autoimmune diseases and in defense against infectious diseases, however, remains largely unknown. Identifying novel antigenic lipids that bind to CD1 molecules and understanding the role of CD1-restricted T cells should lead to the successful development of vaccines, because the lipids can be used as antigens and also as adjuvants. In this paper, we have constructed functional recombinant human CD1 dimeric proteins and established a competitive ELISA assay to measure the lipid binding to CD1 molecules using the CD1 dimers. By using the competitive ELISA assay, we were able to show that the lipid extracts from murine malaria parasites can actually be loaded onto CD1 molecules. In addition, we have demonstrated that artificial antigen-presenting cells, which consist of magnetic beads coated with CD1d dimer and anti-CD28 antibody, stimulated and expanded human invariant NKT cells as efficiently as autologous immature DCs. A set of the tools presented in the current study should be valuable for screening various CD1 molecule-binding lipid antigens and for isolating CD1-restricted T cells.
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影响因子：
5.4
作者：
Kaser, Arthur;Hava, David L.;Dougan, Stephanie K.;Chen, Zhangguo;Zeissig, Sebastian;Brenner, Michael B.;Blumberg, Richard S.
通讯作者：
Blumberg, Richard S.
影响因子：
30.5
作者：
Kinjo, Yuki;Tupin, Emmanuel;Kronenberg, Mitchell
通讯作者：
Kronenberg, Mitchell
不变的T细胞受体α链由小鼠和人类的主要组织相容性复合物I类CD4+和CD4-8-T细胞的独特子集使用。
影响因子：
15.3
作者：
Lantz, Olivier;Bendelac, Albert
通讯作者：
Bendelac, Albert
影响因子：
4.1
作者：
Marchesini, N;Luo, SH;Docampo, R
通讯作者：
Docampo, R
DOI：
10.1073/pnas.90.14.6671
发表时间：
1993-07-15
影响因子：
11.1
作者：
DALPORTO, J;JOHANSEN, TE;SCHNECK, JP
通讯作者：
SCHNECK, JP