Identifying and tracking proteins through the marine water column: insights into the inputs and preservation mechanisms of protein in sediments.
Identifying and tracking proteins through the marine water column: insights into the inputs and preservation mechanisms of protein in sediments.
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DOI：
10.1016/j.gca.2012.01.002
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发表时间：
2012-04-15
影响因子：
5
通讯作者：
Harvey, H. Rodger
中科院分区：
文献类型：
--
作者：
Moore, Eli K.;Nunn, Brook L.;Goodlett, David R.;Harvey, H. Rodger
初级生产过程中产生的蛋白质是海洋有机氮和碳的重要组成部分，并有可能提供环境中生物体的特定信息。白令海是一个以季节性水华为主的高产系统，被用作藻类蛋白质的模型系统，通过水柱跟踪并纳入碎屑沉积材料。在白令海大陆架和深海盆表层沉积物的多个深度沿着采集了悬浮颗粒和沉降颗粒样品。修改后的标准蛋白质组制剂与高压液相色谱-串联质谱法结合使用，以确定存在的蛋白质组，并监测其分布的变化。在地表水沃茨207蛋白质被确定，通过水柱减少到52蛋白质确定后，盛开的货架表层沉积物和24蛋白质在更深（3490米）盆地沉积物。在所有样品中鉴定的蛋白质绝大多数是硅藻的起源，反映了它们在春季水华期间对生物量的主要贡献。鉴定的蛋白质主要来自代谢、结合/结构和转运相关蛋白质组。显着的线性相关性之间观察到的蛋白质的数量和总水解氨基酸的碳和氮标准化的浓度。细胞器结合，跨膜，光合作用，和其他蛋白质参与光捕获优先保留在回收过程中。这些研究结果表明，细胞器和膜保护的重要机制，提高运输和纳入沉积物中的蛋白质的保存。
Proteins generated during primary production represent an important fraction of marine organic nitrogen and carbon, and have the potential to provide organism-specific information in the environment. The Bering Sea is a highly productive system dominated by seasonal blooms and was used as a model system for algal proteins to be tracked through the water column and incorporated into detrital sedimentary material. Samples of suspended and sinking particles were collected at multiple depths along with surface sediments on the continental shelf and deeper basin of the Bering Sea. Modified standard proteomic preparations were used in conjunction with high pressure liquid chromatography-tandem mass spectrometry to identify the suite of proteins present and monitor changes in their distribution. In surface waters 207 proteins were identified, decreasing through the water column to 52 proteins identified in post-bloom shelf surface sediments and 24 proteins in deeper (3490 m) basin sediments. The vast majority of identified proteins in all samples were diatom in origin, reflecting their dominant contribution of biomass during the spring bloom. Identified proteins were predominantly from metabolic, binding/structural, and transport-related protein groups. Significant linear correlations were observed between the number of proteins identified and the concentration of total hydrolysable amino acids normalized to carbon and nitrogen. Organelle-bound, transmembrane, photosynthetic, and other proteins involved in light harvesting were preferentially retained during recycling. These findings suggest that organelle and membrane protection represent important mechanisms that enhance the preservation of protein during transport and incorporation into sediments.
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影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
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影响因子：
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作者：
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