De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes.
De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes.
复制标题
亚洲玉米螟（Ostriniafurnacalis）的从头转录组和表达谱分析揭示了相关的氟虫酰胺反应基因
DOI：
10.1186/s12864-016-3431-6
复制
发表时间：
2017-01-05
期刊：
影响因子：
4.4
通讯作者：
Yuan H
中科院分区：
文献类型：
--
作者：
Cui L;Rui C;Yang D;Wang Z;Yuan H
亚洲玉米螟（Asian corn borer，ACB）是亚洲地区危害最大的玉米害虫。然而，基因组或转录组信息的缺乏严重阻碍了我们在分子水平和全基因组范围内对ACB各个方面的进一步了解。在这里，我们使用Ion Torrent个人基因组机器（PGM）测序仪来探索ACB转录组，并识别响应氟虫双胺的相关基因，显示出对ACB的高选择性活性。我们获得了35，430个unigenes，平均长度为716 bp，代表了现有ACB可用cDNA序列的急剧扩展。这些序列用非冗余蛋白（Nr）、基因本体（GO）、同源群簇（COG）和京都基因和基因组百科全书（KEGG）注释以更好地理解它们的功能。人工构建了31种细胞色素P450单加氧酶（P450 s）、27种羧基/胆碱酯酶（CCEs）和19种谷胱甘肽S-转移酶（GSTs）的系统发育树，并鉴定了25种编码靶蛋白（乙酰胆碱酯酶、烟碱乙酰胆碱受体、γ-氨基丁酸受体、谷氨酸门控氯离子通道、电压门控钠离子通道和兰尼碱受体）的单基因。此外，我们比较并验证了氟虫双胺处理后差异表达的unigenes，揭示了解毒酶基因（P450和酯酶）、钙信号通路和肌肉控制通路（tickin和tropomyosin），免疫球蛋白（hemolin），化学感受蛋白和热休克蛋白70在氟虫双胺的反应中显著过表达，而表皮蛋白，蛋白酶和氧化还原酶显示低得多的表达水平。所获得的转录组信息为ACB的进一步研究提供了大量的基因组资源。差异表达基因数据将阐明ACB对新型双酰胺类杀虫剂氟虫双酰胺的分子机制。特别是，这些发现将有助于鉴定与杀虫剂抗性有关的基因，并开发新的化合物来控制ACB。本文的在线版本（doi：10.1186/s12864-016-3431-6）包含补充材料，可供授权用户使用。
The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has become the most damaging insect pest of corn in Asia. However, the lack of genome or transcriptome information heavily hinders our further understanding of ACB in every aspect at a molecular level and on a genome-wide scale. Here, we used the Ion Torrent Personal Genome Machine (PGM) Sequencer to explore the ACB transcriptome and to identify relevant genes in response to flubendiamide, showing high selective activity against ACB. We obtained 35,430 unigenes, with an average length of 716 bp, representing a dramatic expansion of existing cDNA sequences available for ACB. These sequences were annotated with Non-redundant Protein (Nr), Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to better understand their functions. A total of 31 cytochrome P450 monooxygenases (P450s), 27 carboxyl/cholinesterases (CCEs) and 19 glutathione S-transferases (GSTs) were manually curated to construct phylogenetic trees, and 25 unigenes encoding target proteins (acetylcholinesterase, nicotinic acetylcholine receptor, gamma-aminobutyric acid receptor, glutamate-gated chloride channel, voltage-gated sodium channel and ryanodine receptor) were identified. In addition, we compared and validated the differentially expressed unigenes upon flubendiamide treatment, revealing that the genes for detoxification enzymes (P450s and esterase), calcium signaling pathways and muscle control pathways (twitchin and tropomyosin), immunoglobulin (hemolin), chemosensory protein and heat shock protein 70 were significantly overexpressed in response to flubendiamide, while the genes for cuticular protein, protease and oxidoreductase showed much lower expression levels. The obtained transcriptome information provides large genomic resources available for further studies of ACB. The differentially expressed gene data will elucidate the molecular mechanisms of ACB in response to the novel diamide insecticide, flubendiamide. In particular, these findings will facilitate the identification of the genes involved in insecticide resistance and the development of new compounds to control the ACB. The online version of this article (doi:10.1186/s12864-016-3431-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
影响因子：
2.8
作者：
Folcher, Laurent;Jarry, Marc;Regnault-Roger, Catherine
通讯作者：
Regnault-Roger, Catherine
影响因子：
4.2
作者：
Jin T;Chang X;Gatehouse AM;Wang Z;Edwards MG;He K
通讯作者：
He K
鉴定在小地老虎性信息素腺中表达的基因在性信息素生物合成和运输中的推定作用
影响因子：
4.4
作者：
Gu SH;Wu KM;Guo YY;Pickett JA;Field LM;Zhou JJ;Zhang YJ
通讯作者：
Zhang YJ
影响因子：
4.7
作者：
Li, Fanchi;Ni, Min;Li, Bing
通讯作者：
Li, Bing