Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport.
Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport.
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鉴定在小地老虎性信息素腺中表达的基因在性信息素生物合成和运输中的推定作用
DOI：
10.1186/1471-2164-14-636
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发表时间：
2013-09-22
期刊：
影响因子：
4.4
通讯作者：
Zhang YJ
中科院分区：
文献类型：
--
作者：
Gu SH;Wu KM;Guo YY;Pickett JA;Field LM;Zhou JJ;Zhang YJ
昆虫化学生态学的挑战之一是了解昆虫信息素的合成、检测和降解。通过比较测序和基因特异性表达谱的全基因组调查为这一挑战提供了丰富的资源。A.害虫是许多作物的破坏性害虫，进一步鉴定参与信息素生物合成和运输的基因可以为破坏其化学通讯和作物保护提供潜在的靶标。在这里，我们报告了454个下一代A。昆虫信息素腺体转录组，参与信息素产生、运输和降解的基因的鉴定和表达谱。转录组分析共获得23473个单基因，其中86%为A.依地而定。42个转录本编码参与信息素生物合成的酶，其中15个特异性地或主要地在信息素腺体中表达，其表达水平比体内高5至120倍。两个转录本编码的脂肪酸合成酶和去饱和酶是非常丰富的转录组和表达超过40倍，在腺体比在身体。编码2乙酰辅酶A羧化酶，1脂肪酸合成酶，2去饱和酶，3酰基辅酶A还原酶，2醇氧化酶，2醛还原酶和3乙酰转移酶的转录本在信息素腺体中的表达水平显着高于体内。17个酯酶转录本不具有腺体特异性，其中7个在触角中高表达。7个转录编码气味结合蛋白（OBP）和8个编码化学感受蛋白（CSP）进行了鉴定。两个CSP转录物（AipsCSP 2、AipsCSP 8）在信息素腺体转录组中高度丰富，并且这通过qRT-PCR证实。其中1个OBP（AipsOBP 6）富含信息素腺体，3个OBP（AipsOBP 1、AipsOBP 2和AipsOBP 4）富含触角。在此基础上提出了可能的A.主要和次要性信息素组分的异源生物合成途径。我们的研究确定了可能参与性信息素生物合成和运输的基因。当然。这些基因可能在性信息素的产生、运输和降解过程中发挥重要作用，并可能作为干扰性信息素释放的靶基因。在性信息素腺体中高表达的CSP和OBP的鉴定表明，它们可能在性信息素产生过程中参与性信息素的结合、运输和释放。鳞翅目昆虫和其他鳞翅目昆虫。
One of the challenges in insect chemical ecology is to understand how insect pheromones are synthesised, detected and degraded. Genome wide survey by comparative sequencing and gene specific expression profiling provide rich resources for this challenge. A. ipsilon is a destructive pest of many crops and further characterization of the genes involved in pheromone biosynthesis and transport could offer potential targets for disruption of their chemical communication and for crop protection. Here we report 454 next-generation sequencing of the A. ipsilon pheromone gland transcriptome, identification and expression profiling of genes putatively involved in pheromone production, transport and degradation. A total of 23473 unigenes were obtained from the transcriptome analysis, 86% of which were A. ipsilon specific. 42 transcripts encoded enzymes putatively involved in pheromone biosynthesis, of which 15 were specifically, or mainly, expressed in the pheromone glands at 5 to 120-fold higher levels than in the body. Two transcripts encoding for a fatty acid synthase and a desaturase were highly abundant in the transcriptome and expressed more than 40-fold higher in the glands than in the body. The transcripts encoding for 2 acetyl-CoA carboxylases, 1 fatty acid synthase, 2 desaturases, 3 acyl-CoA reductases, 2 alcohol oxidases, 2 aldehyde reductases and 3 acetyltransferases were expressed at a significantly higher level in the pheromone glands than in the body. 17 esterase transcripts were not gland-specific and 7 of these were expressed highly in the antennae. Seven transcripts encoding odorant binding proteins (OBPs) and 8 encoding chemosensory proteins (CSPs) were identified. Two CSP transcripts (AipsCSP2, AipsCSP8) were highly abundant in the pheromone gland transcriptome and this was confirmed by qRT-PCR. One OBP (AipsOBP6) were pheromone gland-enriched and three OBPs (AipsOBP1, AipsOBP2 and AipsOBP4) were antennal-enriched. Based on these studies we proposed possible A. ipsilon biosynthesis pathways for major and minor sex pheromone components. Our study identified genes potentially involved in sex pheromone biosynthesis and transport in A. ipsilon. The identified genes are likely to play essential roles in sex pheromone production, transport and degradation and could serve as targets to interfere with pheromone release. The identification of highly expressed CSPs and OBPs in the pheromone gland suggests that they may play a role in the binding, transport and release of sex pheromones during sex pheromone production in A. ipsilon and other Lepidoptera insects.
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影响因子：
3.7
作者：
Du M;Yin X;Zhang S;Zhu B;Song Q;An S
通讯作者：
An S
影响因子：
3.7
作者：
Hagström AK;Liénard MA;Groot AT;Hedenström E;Löfstedt C
通讯作者：
Löfstedt C
影响因子：
2.3
作者：
BJOSTAD, LB;ROELOFS, WL
通讯作者：
ROELOFS, WL
DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
影响因子：
2.3
作者：
Gemeno, C;Haynes, KF
通讯作者：
Haynes, KF