Gene expression data support the hypothesis that Isoetes rootlets are true roots and not modified leaves.
Gene expression data support the hypothesis that Isoetes rootlets are true roots and not modified leaves.
复制标题
基因表达数据支持Isoetes rootlets是真正的根而不是修饰的叶的假设。
DOI：
10.1038/s41598-020-78171-y
复制
发表时间：
2020-12-09
影响因子：
4.6
通讯作者：
Dolan L
中科院分区：
文献类型：
--
作者：
Hetherington AJ;Emms DM;Kelly S;Dolan L
根生石松是陆地植物群，包括第一个在地球上生长的巨型树木和现存的Isoclonal属物种。两个相互排斥的假设占终端生根轴称为小根之间的根状石松类的演变。一种假说认为细根是真正的根，就像其他石松科植物的根一样。另一种说法是rootlet是经过修改的叶子。在这里，我们通过研究棘孢等鞭金藻的叶和根中的基因表达来测试每个假设的预测。我们组装了无菌培养的I.棘孢菌属。基因表达特征I.棘孢根和叶的差异。此外，I.棘孢小根的基因表达特征与卷柏和拟南芥真根的基因表达特征相似。RSL基因在根中的细胞分化中起正调控作用，它们在I. echinospora rootlets，S. moellendorffii roots和A.与每个相应物种的叶子相比，thaliana根。总之，来自I.棘孢属的植物与Isopropylenetetrium的小根是真正的根而不是修饰的叶的假设是一致的。
Rhizomorphic lycopsids are the land plant group that includes the first giant trees to grow on Earth and extant species in the genus Isoetes. Two mutually exclusive hypotheses account for the evolution of terminal rooting axes called rootlets among the rhizomorphic lycopsids. One hypothesis states that rootlets are true roots, like roots in other lycopsids. The other states that rootlets are modified leaves. Here we test predictions of each hypothesis by investigating gene expression in the leaves and rootlets of Isoetes echinospora. We assembled the de novo transcriptome of axenically cultured I. echinospora. Gene expression signatures of I. echinospora rootlets and leaves were different. Furthermore, gene expression signatures of I. echinospora rootlets were similar to gene expression signatures of true roots of Selaginella moellendorffii and Arabidopsis thaliana. RSL genes which positively regulate cell differentiation in roots were either exclusively or preferentially expressed in the I. echinospora rootlets, S. moellendorffii roots and A. thaliana roots compared to the leaves of each respective species. Taken together, gene expression data from the de-novo transcriptome of I. echinospora are consistent with the hypothesis that Isoetes rootlets are true roots and not modified leaves.
登录
查看更多内容
影响因子：
3.7
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
通讯作者：
OJIMA, K
影响因子：
1.9
作者：
BATEMAN, RM;DIMICHELE, WA;WILLARD, DA
通讯作者：
WILLARD, DA
影响因子：
0.8
作者：
Pigg, KB
通讯作者：
Pigg, KB
影响因子：
1.8
作者：
Caldeira, Cecilio Frois;Abranches, Cinthia Bandeira;Siqueira, Jose Oswaldo
通讯作者：
Siqueira, Jose Oswaldo
影响因子：
1.9
作者：
EGGERT, DA
通讯作者：
EGGERT, DA