Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice.
Transgenic expression of dentin phosphoprotein (DPP) partially rescued the dentin defects of DSPP-null mice.
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DOI：
10.1371/journal.pone.0195854
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Qin C
中科院分区：
文献类型：
--
作者：
Zhang H;Xie X;Liu P;Liang T;Lu Y;Qin C
牙本质唾液磷蛋白（DSPP）基因突变导致牙本质发育不全。合成后，DSPP被蛋白水解成NH2-和cooh -末端片段。DSPP的nh2末端片段高度糖基化但不被磷酸化，而cooh末端片段（称为“牙本质磷酸化蛋白”或“DPP”）高度磷酸化但不被糖基化。这两个片段被认为在牙本质形成中起着不同的作用。为了分析DPP在牙本质形成中的作用，我们创建了“Dspp-/-；DPP Tg小鼠”，表达由I型胶原启动子驱动的转基因DPP，但缺乏内源性Dspp基因。我们对Dspp-/-的牙本质进行了表征；DPP Tg小鼠采用x线摄影、组织学、扫描电镜、双荧光标记、免疫组织化学和原位杂交。显微计算机断层扫描分析显示，在出生后6个月，转基因DPP的表达使dspp缺失小鼠的牙本质厚度增加了97.1%，牙本质物质密度恢复了29.5%。组织学分析显示，Dspp-/-缺失小鼠的前牙本质异常变宽，Dspp-/-缺失小鼠的前牙本质异常变宽；DPP - Tg小鼠比无dspp小鼠窄。扫描电镜分析显示，Dspp-/-的牙本质小管；DPP - Tg小鼠比Dspp-null小鼠组织更好。双荧光标记分析表明，牙本质矿物沉积速率在Dspp-/-；与无dspp小鼠相比，DPP Tg明显改善。上述结果表明，DPP转基因表达部分修复了DSPP缺失小鼠的牙本质缺陷，提示DPP可能促进牙本质的形成，DPP与DSPP nh2末端片段的协同作用可能是牙本质形成的必要条件。
Mutations in the dentin sialophosphoprotein (DSPP) gene cause dentinogenesis imperfecta. After synthesis, DSPP is proteolytically processed into NH2- and COOH-terminal fragments. The NH2-terminal fragment of DSPP is highly glycosylated but not phosphorylated, whereas the COOH-terminal fragment (named “dentin phosphoprotein” or “DPP”) is highly phosphorylated but not glycosylated. These two fragments are believed to perform distinct roles in dentin formation. To analyze the functions of DPP in dentinogenesis, we created “Dspp-/-;DPP Tg mice”, which expressed transgenic DPP driven by a Type I collagen promoter but lacked the endogenous Dspp gene. We characterized the dentin of the Dspp-/-;DPP Tg mice using X-ray radiography, histology, scanning electron microscopy, double fluorochrome labeling, immunohistochemistry and in situ hybridization. Micro-computed tomography analyses revealed that at postnatal 6 months, the transgenic expression of DPP increased the dentin thickness of the Dspp-null mice by 97.1% and restored the dentin material density by 29.5%. Histological analyses showed that the Dspp-null mice manifested an abnormal widening of the predentin while the predentin in Dspp-/-;DPP Tg mice was narrower than in the Dspp-null mice. Scanning electron microscopy analyses showed that the dentinal tubules in the Dspp-/-;DPP Tg mice were better organized than in the Dspp-null mice. The double fluorochrome labeling analyses demonstrated that the dentin mineral deposition rate in the Dspp-/-;DPP Tg mice was significantly improved compared to that in the Dspp-null mice. These findings indicate that the transgenic expression of DPP partially rescued the dentin defects of the DSPP-null mice, suggesting that DPP may promote dentin formation and that the coordinated actions between DPP and the NH2-terminal fragment of DSPP may be necessary for dentinogenesis.
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影响因子：
30.8
作者：
Xiao, SX;Yu, C;Kong, XY
通讯作者：
Kong, XY
影响因子：
30.8
作者：
Zhang, XH;Zhao, J;Shen, Y
通讯作者：
Shen, Y
DOI：
10.1016/s0934-8832(11)80030-2
发表时间：
1992-11-01
期刊：
MATRIX
影响因子：
--
作者：
BUTLER, WT;BHOWN, M;VANDIJK, S
通讯作者：
VANDIJK, S
影响因子：
4.2
作者：
LINDE, A;LUSSI, A;CRENSHAW, MA
通讯作者：
CRENSHAW, MA
影响因子：
4.8
作者：
Sreenath, T;Thyagarajan, T;Kulkarni, AB
通讯作者：
Kulkarni, AB