Genetically-determined variations in photosynthesis indicate roles for specific fatty acid species in chilling responses
Genetically-determined variations in photosynthesis indicate roles for specific fatty acid species in chilling responses
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DOI：
10.1111/pce.14313
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发表时间：
2022-03-28
影响因子：
7.3
通讯作者：
Kramer，David M.
中科院分区：
文献类型：
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作者：
Hoh，Donghee;Horn，Patrick J.;Kramer，David M.
使用一群重组自交系 (RIL) 豇豆 (Vigna unguiculata. L. Walp)，我们测试了脂质含量与光合作用的低温反应之间的相互联系。在低温条件下（19°C/13°C，白天/夜间），我们观察到光合光反应的数量性状位点区间与特定脂肪酸之间的关联，最引人注目的是仅在磷脂酰甘油（PG 16:1t）中发现的类囊体特异性脂肪酸16:1Δ3trans。相比之下，我们没有观察到与大量多不饱和脂肪酸或高熔点 PG（PG 16:0、PG 18:0 和 PG 16:1t 之和）的共关联，而此前人们认为这些脂肪酸与寒冷敏感性有关。这些结果表明，豇豆的冷冻敏感性是通过特定的脂质相互作用而不是整体特性来调节的。我们能够使用突变体和转基因拟南芥品系来概括 PG 16:1t 水平对低温下光合作用反应的预测影响。由于 PG 16:1t 的合成需要过氧化还原蛋白-Q 的活性，而过氧化还原蛋白-Q 被 H2O2 激活，并且已知参与氧化还原信号传导，因此我们假设 PG 16:1t 的积累是由于光合作用的上游影响而发生的，这些影响改变了氧化还原状态和活性氧的产生。
Using a population of recombinant inbred lines (RILs) cowpea (Vigna unguiculata. L. Walp), we tested for co‐linkages between lipid contents and chilling responses of photosynthesis. Under low‐temperature conditions (19°C/13°C, day/night), we observed co‐linkages between quantitative trait loci intervals for photosynthetic light reactions and specific fatty acids, most strikingly, the thylakoid‐specific fatty acid 16:1Δ3transfound exclusively in phosphatidylglycerol (PG 16:1t). By contrast, we did not observe co‐associations with bulk polyunsaturated fatty acids or high‐melting‐point‐PG (sum of PG 16:0, PG 18:0 and PG 16:1t) previously thought to be involved in chilling sensitivity. These results suggest that in cowpea, chilling sensitivity is modulated by specific lipid interactions rather than bulk properties. We were able to recapitulate the predicted impact of PG 16:1t levels on photosynthetic responses at low temperature using mutants and transgenic Arabidopsis lines. Because PG 16:1t synthesis requires the activity of peroxiredoxin‐Q, which is activated by H2O2and known to be involved in redox signalling, we hypothesise that the accumulation of PG 16:1t occurs as a result of upstream effects on photosynthesis that alter redox status and production of reactive oxygen species.
影响因子：
5.6
作者：
Hara S;Hisabori T
通讯作者：
Hisabori T