Vertebrate mRNAs with a 5'-terminal pyrimidine tract are candidates for translational repression in quiescent cells: characterization of the translational cis-regulatory element
Vertebrate mRNAs with a 5'-terminal pyrimidine tract are candidates for translational repression in quiescent cells: characterization of the translational cis-regulatory element
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具有 5 末端嘧啶束的脊椎动物 mRNA 是静止细胞中翻译抑制的候选者：翻译顺式调控元件的表征
DOI：
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发表时间：
1994
影响因子：
5.3
通讯作者：
O. Meyuhas
中科院分区：
文献类型：
--
作者：
Dror Avni;Silvian Shama;F. Loreni;O. Meyuhas
哺乳动物核糖体蛋白 (rp) mRNA 的翻译在非生长细胞中被选择性抑制。这种反应是通过这些 mRNA 5' 非翻译区中的调节元件介导的，并包括 5' 末端寡嘧啶束 (5' TOP)。为了进一步表征翻译顺式调控元件，我们监测了来自转染嵌合基因的各种内源和异源mRNA或杂合转录物的翻译行为。在正常生长或在各种生理条件下生长停滞的细胞中评估这些 mRNA 的翻译效率。我们的实验得到了以下结果：（i）哺乳动物 rp mRNA 的翻译在两栖动物细胞中受到适当调节，同样，两栖动物 rp mRNA 在哺乳动物细胞中受到调节，表明控制 rp mRNA 翻译所需的所有元件在脊椎动物中都是保守的； (ii) 选择性翻译控制不限于 rp mRNA，因为编码天然存在的泛素-rp 融合蛋白和延伸因子 1 α（包含 5' TOP）的 mRNA 也符合这种调节模式； (iii) 大鼠 rpP2 mRNA 在其 5' 顶部仅含有 5 个嘧啶，但该 mRNA 以与 5' 顶部具有 8 个或更多嘧啶的其他 rp mRNA 相同的方式进行翻译控制； (iv) 这种调节模式的充分体现似乎需要 5' TOP 和紧邻下游的序列； (v) 来自 rpL32 mRNA 的完整翻译调控元件即使前面有一个 A 残基也无法发挥其调控特性。
The translation of mammalian ribosomal protein (rp) mRNAs is selectively repressed in nongrowing cells. This response is mediated through a regulatory element residing in the 5' untranslated region of these mRNAs and includes a 5' terminal oligopyrimidine tract (5' TOP). To further characterize the translational cis-regulatory element, we monitored the translational behavior of various endogenous and heterologous mRNAs or hybrid transcripts derived from transfected chimeric genes. The translational efficiency of these mRNAs was assessed in cells that either were growing normally or were growth arrested under various physiological conditions. Our experiments have yielded the following results: (i) the translation of mammalian rp mRNAs is properly regulated in amphibian cells, and likewise, amphibian rp mRNA is regulated in mammalian cells, indicating that all of the elements required for translation control of rp mRNAs are conserved among vertebrate classes; (ii) selective translational control is not confined to rp mRNAs, as mRNAs encoding the naturally occurring ubiquitin-rp fusion protein and elongation factor 1 alpha, which contain a 5' TOP, also conform this mode of regulation; (iii) rat rpP2 mRNA contains only five pyrimidines in its 5' TOP, yet this mRNA is translationally controlled in the same fashion as other rp mRNAs with a 5' TOP of eight or more pyrimidines; (iv) full manifestation of this mode of regulation seems to require both the 5' TOP and sequences immediately downstream; and (v) an intact translational regulatory element from rpL32 mRNA fails to exert its regulatory properties even when preceded by a single A residue.
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成肌细胞分化和体外介导小鼠 S16 核糖体蛋白 mRNA 翻译的序列以及体外翻译的可能控制点。
影响因子：
10.5
作者：
Hammond,ML;Merrick,W;Bowman,LH
通讯作者：
Bowman,LH
DOI：
10.1073/pnas.90.16.7642
发表时间：
1993-08-15
影响因子：
11.1
作者：
HELLEN, CUT;WITHERELL, GW;WIMMER, E
通讯作者：
WIMMER, E
DOI：
10.1016/s0021-9258(19)39637-1
发表时间：
1990-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Kaspar;W. Rychlik;M. White;R. Rhoads;D. Morris
通讯作者：
R. Kaspar;W. Rychlik;M. White;R. Rhoads;D. Morris
DOI：
10.1016/s0021-9258(19)34036-0
发表时间：
1990-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Elizabeth C. Theil
通讯作者：
Elizabeth C. Theil
含有内含子并编码大鼠核糖体蛋白 P2 的基因的结构。
影响因子：
14.9
作者：
Chan,YL;Wool,IG
通讯作者：
Wool,IG