GC × GC–TOFMS and supervised multivariate approaches to study human cadaveric decomposition olfactive signatures
GC × GC–TOFMS and supervised multivariate approaches to study human cadaveric decomposition olfactive signatures
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GC × GC-TOFMS 和监督多元方法研究人类尸体分解嗅觉特征
DOI：
10.1007/s00216-015-8683-5
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发表时间：
2015
影响因子：
4.3
通讯作者：
J. Focant
中科院分区：
文献类型：
--
作者：
Pierre‐Hugues Stefanuto;Katelynn A. Perrault;S. Stadler;Romain Pesesse;H. LeBlanc;S. Forbes;J. Focant
在法医死亡化学中，对软组织分解过程的认识仍然有限。更好地了解分解过程和相关挥发性有机化合物（VOC）的特征可以帮助提高受害者恢复（VR）犬的训练，这些犬在自然灾害中用于搜索被困的受害者或在刑事调查中定位尸体。矩阵的复杂性和这一过程的动态性要求使用综合分析方法进行调查。此外，环境和个人之间的可变性在正常化方面造成了额外的困难。与传统的一维气相色谱法（1DGC）相比，综合二维气相色谱法（GC × GC）可以提高腐烂尸体散发的复杂VOCs混合物的分辨率。本研究将GC × GC耦合飞行时间质谱（TOFMS）的分析优势与监督多元统计的数据处理稳健性相结合，研究了人体遗骸分解早期挥发性有机化合物的特征。比较了各种监督多元方法来解释大数据集。此外，还监测了猪尸体的早期分解阶段（通常在实地研究中用作人类替代品），以获得两种VOC概况的直接比较，并估计这种人类分解模拟模型的稳健性。在这项研究中，我们证明了猪和人的分解过程可以用软组织分解早期产生的主要化合物的相同趋势来描述。
In forensic thanato-chemistry, the understanding of the process of soft tissue decomposition is still limited. A better understanding of the decomposition process and the characterization of the associated volatile organic compounds (VOC) can help to improve the training of victim recovery (VR) canines, which are used to search for trapped victims in natural disasters or to locate corpses during criminal investigations. The complexity of matrices and the dynamic nature of this process require the use of comprehensive analytical methods for investigation. Moreover, the variability of the environment and between individuals creates additional difficulties in terms of normalization. The resolution of the complex mixture of VOCs emitted by a decaying corpse can be improved using comprehensive two-dimensional gas chromatography (GC × GC), compared to classical single-dimensional gas chromatography (1DGC). This study combines the analytical advantages of GC × GC coupled to time-of-flight mass spectrometry (TOFMS) with the data handling robustness of supervised multivariate statistics to investigate the VOC profile of human remains during early stages of decomposition. Various supervised multivariate approaches are compared to interpret the large data set. Moreover, early decomposition stages of pig carcasses (typically used as human surrogates in field studies) are also monitored to obtain a direct comparison of the two VOC profiles and estimate the robustness of this human decomposition analog model. In this research, we demonstrate that pig and human decomposition processes can be described by the same trends for the major compounds produced during the early stages of soft tissue decomposition.
DOI：
10.1016/j.jchromb.2012.05.038
发表时间：
2012-07-15
影响因子：
3
作者：
Bean, Heather D.;Dimandja, Jean-Marie D.;Hill, Jane E.
通讯作者：
Hill, Jane E.