Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.
Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.
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DOI：
10.1016/j.jchromb.2012.05.038
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发表时间：
2012-07-15
影响因子：
3
通讯作者：
Hill, Jane E.
中科院分区：
文献类型：
--
作者：
Bean, Heather D.;Dimandja, Jean-Marie D.;Hill, Jane E.
细菌产生独特的挥发性混合物，可用于识别该物种的传染性病原体，并可能确定菌株水平。然而，由于人类病原体的种类繁多，以及这些细菌中的一些细菌的密切相关性，基于其挥发性代谢物组的细菌的稳健鉴定可能需要每个物种的大量挥发性化合物。采用全二维气相色谱-飞行时间质谱（GC×GC-TOFMS）技术对铜绿假单胞菌PA 14在溶原肉汤中生长24 h后的顶空挥发物进行了鉴定。这是首次报道使用GC×GC-TOFMS表征细菌顶空挥发物。该色谱方法提供的分析纯度有助于鉴定28种新的铜绿假单胞菌衍生挥发物，几乎使该种属的挥发物列表增加了一倍。
Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC-TOFMS) to the identification of the headspace volatiles of P. aeruginosa PA14 grown for 24 h in lysogeny broth. This is the first reported use of GC×GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species.
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影响因子：
9.4
作者：
Zhu, Jiangjiang;Bean, Heather D.;Hill, Jane E.
通讯作者：
Hill, Jane E.
影响因子：
2
作者：
Chippendale, Thomas W. E.;Spanel, Patrik;Smith, David
通讯作者：
Smith, David
影响因子：
9.4
作者：
LABOWS, JN;MCGINLEY, KJ;LEYDEN, JJ
通讯作者：
LEYDEN, JJ
影响因子：
7.4
作者：
Dimandja, JMD
通讯作者：
Dimandja, JMD
影响因子：
8.8
作者：
Moularat, Stephane;Robine, Enric;Oturan, Mehmet A.
通讯作者：
Oturan, Mehmet A.