T lymphocyte subset imbalances in patients contribute to ankylosing spondylitis.
T lymphocyte subset imbalances in patients contribute to ankylosing spondylitis.
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DOI：
10.3892/etm.2014.2046
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发表时间：
2015-01
影响因子：
2.7
通讯作者：
Zhong D
中科院分区：
文献类型：
--
作者：
Wang C;Liao Q;Hu Y;Zhong D
强直性脊柱炎是一种慢性炎症性风湿性疾病，其特征是脊柱和骶髂关节的炎症。迄今为止，该病的病因尚不清楚。本研究探讨了T淋巴细胞亚群变化与强直性脊柱炎进展的相关性。共有 55 名强直性脊柱炎患者（其中重度病例 22 例，轻症病例 23 例）和 20 名健康个体被纳入研究。首先收集病灶内穿刺细胞和血清，制备淋巴细胞和外周血单个核细胞。其次，通过定量PCR、ELISA和流式细胞术分析检测一系列免疫球蛋白、补体、辅助性T细胞、细胞毒性T细胞、调节细胞和细胞因子的水平。强直性脊柱炎患者的α-球蛋白、γ-球蛋白、免疫球蛋白(Ig)G、IgA、IgM、血清补体C3和补体C4的表达水平显着升高。此外，与健康个体相比，强直性脊柱炎组（轻度和重度）的 Th1 和 Th17 细胞百分比显着更高。结果，强直性脊柱炎患者的 Th1/Th2 和 Th17/Treg 比率显着升高。此外，T淋巴细胞亚群比例失衡导致免疫介质表达增加，包括干扰素（IFN）-γ和白细胞介素（IL）-17A。与对照组相比，强直性脊柱炎组的 IFN-γ 和 IL-17A mRNA 和蛋白表达水平较高。本研究为T淋巴细胞亚群的功能和潜在机制提供了进一步的证据，可能有助于强直性脊柱炎的诊断和治疗。
Ankylosing spondylitis is a chronic inflammatory rheumatic disease, which is characterized by inflammation of the spine and the sacroiliac joints. To date, the disease etiology remains unclear. In the present study, the correlation of T lymphocyte subset changes with the progression of ankylosing spondylitis was investigated. A total of 55 patients with ankylosing spondylitis (22 severe and 23 mild cases) and 20 healthy individuals were selected. Firstly, the punctured cells in the lesions and the serum were collected, and the lymphocytes and the peripheral blood mononuclear cells were prepared. Secondly, quantitative PCR, ELISA and flow cytometry analyses were carried out to detect the levels of a series of immunoglobulins, complements, helper T cells, cytotoxic T cells, regulatory cells and cytokines. The expression levels of α-globulin, γ-globulin, immunoglobulin (Ig)G, IgA, IgM, serum complement C3, and complement C4 were found to be significantly increased in ankylosing spondylitis patients. In addition, the percentage of Th1 and Th17 cells was found to be significantly higher in the ankylosing spondylitis groups (mild and severe) compared with the healthy individuals. As a result, the Th1/Th2 and Th17/Treg ratios were significantly higher in patients with ankylosing spondylitis. In addition, T lymphocyte subset ratio imbalances contributed to an increased expression of immune mediators, including interferon (IFN)-γ and interleukin (IL)-17A. The mRNA and protein expression levels of IFN-γ and IL-17A were found to be higher in the ankylosing spondylitis groups compared with the control group. The present study provided further evidence on the function and underlying mechanism of T lymphocyte subsets, which may be useful in the diagnosis and treatment of ankylosing spondylitis.
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