Parental age and gene expression profiles in individual human blastocysts.
Parental age and gene expression profiles in individual human blastocysts.
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DOI：
10.1038/s41598-018-20614-8
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发表时间：
2018-02-05
影响因子：
4.6
通讯作者：
Kohda T
中科院分区：
文献类型：
--
作者：
Kawai K;Harada T;Ishikawa T;Sugiyama R;Kawamura T;Yoshida A;Tsutsumi O;Ishino F;Kubota T;Kohda T
基因组的表观遗传状态从受精到植入都是动态变化的。此外，配子发生过程中的生理环境，包括亲本年龄，可能会影响受精后胚胎的表观基因组。从跨代表观遗传学的角度阐明亲代年龄对胚胎基因表达的影响对于改进目前辅助生殖医学中使用的技术是重要的。在这里，我们对通过卵胞浆内单精子注射受精的人胚泡进行了单胚胎RNA-SEQ分析，包括来自相对年长的母亲的囊胚，以阐明父母年龄对胚胎基因表达谱的影响。我们发现了一些基因，这些基因的表达水平随着母亲年龄的增加而降低。在这些基因中，有几个被认为是减数分裂染色体分离的重要基因，如Pttg1、AURKC、SMC1B和MEIKIN。此外，对自噬和胚胎生长至关重要的某些基因的表达水平，特别是GABARAPL1和GABARAPL3，与父亲的高龄呈负相关。此外，来自主要卫星重复序列的转录本水平也随着母亲年龄的增加而下降。这些结果表明，卵母细胞基因组的表观遗传修饰可能会随着亲代年龄的变化而改变，并传递给下一代。
The epigenetic status of the genome changes dynamically from fertilization to implantation. In addition, the physiological environment during the process of gametogenesis, including parental age, may affect the epigenome of the embryo after fertilization. It is important to clarify the influence of parental age on gene expression in the embryo in terms of transgenerational epigenetics to improve the techniques currently used in assisted reproductive medicine. Here, we performed single-embryo RNA-seq analysis on human blastocysts fertilized by intracytoplasmic sperm injection, including from relatively elderly mothers, to elucidate the effects of parental age on the embryonic gene expression profile. We identified a number of genes in which the expression levels were decreased with increasing maternal age. Among these genes, several are considered to be important for meiotic chromosomal segregation, such as PTTG1, AURKC, SMC1B and MEIKIN. Furthermore, the expression levels of certain genes critical for autophagy and embryonic growth, specifically GABARAPL1 and GABARAPL3, were negatively correlated with advanced paternal age. In addition, levels of transcripts derived from major satellite repeats also decreased as the maternal age increased. These results suggest that epigenetic modifications of the oocyte genome may change with parental age and be transmitted to the next generation.
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影响因子：
4
作者：
Munne, S.;Chen, S.;Cohen, J.
通讯作者：
Cohen, J.
影响因子：
9.2
作者：
Revenkova, Ekaterina;Herrmann, Kathleen;Adelfalk, Caroline;Jessberger, Rolf
通讯作者：
Jessberger, Rolf
DOI：
10.5713/ajas.2013.13816
发表时间：
2014-05
期刊：
Asian-Australasian journal of animal sciences
影响因子：
--
作者：
Lee SE;Kim EY;Choi HY;Moon JJ;Park MJ;Lee JB;Jeong CJ;Park SP
通讯作者：
Park SP
影响因子：
4.5
作者：
Balboula AZ;Schindler K
通讯作者：
Schindler K
影响因子：
9.2
作者：
Lister, Lisa Martine;Kouznetsova, Anna;Herbert, Mary
通讯作者：
Herbert, Mary