Oocyte cohesin expression restricted to predictyate stages provides full fertility and prevents aneuploidy.
Oocyte cohesin expression restricted to predictyate stages provides full fertility and prevents aneuploidy.
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DOI：
10.1016/j.cub.2010.08.024
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发表时间：
2010-09-14
期刊：
影响因子：
9.2
通讯作者：
Jessberger, Rolf
中科院分区：
文献类型：
--
作者：
Revenkova, Ekaterina;Herrmann, Kathleen;Adelfalk, Caroline;Jessberger, Rolf
为了确保正确的减数分裂染色体分离，姐妹染色单体凝聚力（SCC）需要保持从出生前在前期I卵母细胞中建立，直到成年卵母细胞成熟后减数分裂持续到中期II。衰老的人类卵母细胞遭受染色体错误分离和非整倍体的急剧增加，这可能是由于通过粘附素的缓慢退化而导致SCC的丧失。这一假说假设胚胎卵母细胞中的粘附素表达足以提供足够的长期SCC。随着年龄的增长，缺乏减数分裂特异性粘附素SMC 1 β的小鼠卵母细胞大量丧失SCC和交叉。为了验证退化假说，我们在出生后不久的原始卵泡阶段特异性且高效地失活了小鼠Smc 1 β基因，此时卵母细胞刚刚进入减数分裂I网状细胞停滞。然而，在成年人中，无论卵母细胞的年龄，交叉位置和SCC是正常的。频率和大小的窝证明完全生育能力，即使在老年女性。因此，SMC 1 β粘附素仅需要在原始卵泡阶段之前的前期I期间表达，以确保SCC直至小鼠的高龄。
To ensure correct meiotic chromosome segregation, sister chromatid cohesion (SCC) needs to be maintained from its establishment in prophase I oocytes before birth until continuation of meiosis into metaphase II upon oocyte maturation in the adult. Aging human oocytes suffer a steep increase in chromosome missegregation and aneuploidy, which may be caused by loss of SCC through slow deterioration of cohesin. This hypothesis assumes cohesin expression in embryonic oocytes is sufficient to provide adequate long-term SCC. With increasing age, mouse oocytes deficient in the meiosis-specific cohesin SMC1β massively lose SCC and chiasmata. To test the deterioration hypothesis, we specifically and highly efficiently inactivated the mouse Smc1β gene at the primordial follicle stage shortly after birth when oocytes had just entered meiosis I dictyate arrest. In the adult, however, irrespective of oocyte age, chiasma positions and SCC are normal. Frequency and size of litters prove full fertility even in aged females. Thus, SMC1β cohesin needs only be expressed during prophase I prior to the primordial follicle stage to ensure SCC up to advanced age of mice.
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DOI：
10.1038/nrg2794
发表时间：
2010-06
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.3
作者：
Revenkova, E;Eijpe, M;Jessberger, R
通讯作者：
Jessberger, R
影响因子：
2.7
作者：
Bristol-Gould, Sarah K.;Kreeger, Pamela K.;Woodruff, Teresa K.
通讯作者：
Woodruff, Teresa K.
影响因子：
21.3
作者：
Revenkova, E;Eijpe, M;Jessberger, R
通讯作者：
Jessberger, R
影响因子：
3.6
作者：
Lan, ZJ;Xu, XP;Cooney, AJ
通讯作者：
Cooney, AJ