Improvement of Curvulamine Production by Precursors Co-addition Strategy in Liquid Culture of Marine-Derived Fungus Curvularia sp. IFB-Z10
Improvement of Curvulamine Production by Precursors Co-addition Strategy in Liquid Culture of Marine-Derived Fungus Curvularia sp. IFB-Z10
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海洋来源真菌弯孢菌液体培养中通过前体共添加策略提高弯孢胺产量。
DOI：
10.1007/s12010-019-03072-4
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发表时间：
2019-07
影响因子：
3
通讯作者：
Yan-Hua Lu
中科院分区：
文献类型：
--
作者：
Xing-Chen Wei;Chang-Qing Liu;Ri-Yu Zhang;Gao-Fan Zheng;Fa-Liang An;Yan-Hua Lu
Curvulamine, a novel scaffold alkaloid with remarkable selective antibacterial activity, is produced by marine fungus Curvularia sp. IFB-Z10. However, its deep pharmaceutical research and application are severely restricted by the low yield, which needs to be solved urgently. The purpose of this study was to improve curvulamine production via precursors co-addition strategy and further reveal the regulation mechanism. In this work, the optimal precursors co-addition conditions were firstly obtained, and curvulamine production achieved 166.74 mg/L with the supply of 250 mg/L alanine and 200 mg/L proline at 60 h, which was 4.08 times that of control. It was observed that under alanine and proline stimulation, fungus exhibited the morphology of a small-diameter compact pellet. Furthermore, the organic acid levels in central carbon metabolism (CCM) were declined with precursors supplement. Besides, precursors also induced the critical biosynthetic gene transcriptions. The above findings collectively promoted curvulamine synthesis. Finally, Curvularia sp. IFB-Z10 fermentation process was successfully established by feeding alanine and proline at 0.021 g/L/h and 0.017 g/L/h rate from 60 to 72 h, and curvulamine production reached 133.58 mg/L in a 5-L bioreactor. The information acquired would facilitate the enhancement of curvulamine yield in submerged fermentation and the research on synthesis regulation of other alkaloids.
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影响因子：
3
作者：
Xiangzhao Mao;Feng Wang;Jianguo Zhang;Shi Chen;Z. Deng;Yaling Shen;D. Wei
通讯作者：
Xiangzhao Mao;Feng Wang;Jianguo Zhang;Shi Chen;Z. Deng;Yaling Shen;D. Wei
影响因子：
3
作者：
Hu, Zhong-Ce;Peng, Li-Yuan;Zheng, Yu-Guo
通讯作者：
Zheng, Yu-Guo
Curvulamine，一种新型抗菌生物碱，包含来自弯孢菌属物种的两个未描述的单位
影响因子：
5.2
作者：
Han, Wen Bo;Lu, Yan Hua;Tan, Ren Xiang
通讯作者：
Tan, Ren Xiang
Ca2 对海洋来源真菌 Curvularia sp 合成 Curvulamine 的诱导作用。
影响因子：
4.4
作者：
Xing-Chen Wei;Chang-Qing Liu;Fa-Liang An;Yan-Hua Lu
通讯作者：
Yan-Hua Lu
影响因子：
4.1
作者：
Xiaoxu Zhang;Hao He;Ying Yin;Weiqiang Zhou;Menghao Cai;Xiangshan Zhou;Yuanxing Zhang
通讯作者：
Xiaoxu Zhang;Hao He;Ying Yin;Weiqiang Zhou;Menghao Cai;Xiangshan Zhou;Yuanxing Zhang