An α-smooth muscle actin (acta2/αsma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells.
An α-smooth muscle actin (acta2/αsma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells.
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DOI：
10.1371/journal.pone.0090590
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Childs SJ
中科院分区：
文献类型：
--
作者：
Whitesell TR;Kennedy RM;Carter AD;Rollins EL;Georgijevic S;Santoro MM;Childs SJ
血管系统的壁细胞包括血管平滑肌细胞（SMC）和周细胞，其作用是稳定血管和/或为血管提供收缩性。脊椎动物壁细胞发育的最早标志物之一是α平滑肌肌动蛋白（acta 2; αsma），由周细胞和SMC表达。目前缺乏斑马鱼中血管壁细胞发育的体内模型，因此我们开发了两种转基因斑马鱼系，其在acta 2表达细胞中驱动GFP或mCherry的表达。这些转基因鱼被用来跟踪胚胎和幼虫转基因斑马鱼的壁细胞的活发育。acta 2：EGFP转基因动物显示出在很大程度上反映天然acta 2表达的表达，早期泛肌肉表达在心肌中开始于24 hpf，随后是骨骼肌和内脏肌。在3.5dpf，在动脉球和腹主动脉中的表达标志着在血管平滑肌中的首次表达。在接下来的10天的发育中，acta 2：EGFP阳性细胞的数量和与壁细胞相关的血管类型的数量增加。有趣的是，壁细胞不运动，一旦它们表达acta 2：EGFP转基因，它们就保持在相同的位置。综上所述，我们的数据表明，斑马鱼壁细胞发育相对较晚，一旦与血管结合，就几乎没有流动性。
Mural cells of the vascular system include vascular smooth muscle cells (SMCs) and pericytes whose role is to stabilize and/or provide contractility to blood vessels. One of the earliest markers of mural cell development in vertebrates is α smooth muscle actin (acta2; αsma), which is expressed by pericytes and SMCs. In vivo models of vascular mural cell development in zebrafish are currently lacking, therefore we developed two transgenic zebrafish lines driving expression of GFP or mCherry in acta2-expressing cells. These transgenic fish were used to trace the live development of mural cells in embryonic and larval transgenic zebrafish. acta2:EGFP transgenic animals show expression that largely mirrors native acta2 expression, with early pan-muscle expression starting at 24 hpf in the heart muscle, followed by skeletal and visceral muscle. At 3.5 dpf, expression in the bulbus arteriosus and ventral aorta marks the first expression in vascular smooth muscle. Over the next 10 days of development, the number of acta2:EGFP positive cells and the number of types of blood vessels associated with mural cells increases. Interestingly, the mural cells are not motile and remain in the same position once they express the acta2:EGFP transgene. Taken together, our data suggests that zebrafish mural cells develop relatively late, and have little mobility once they associate with vessels.
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DOI：
10.1073/pnas.97.21.11403
发表时间：
2000-10-10
影响因子：
11.1
作者：
Kawakami, K;Shima, A;Kawakami, N
通讯作者：
Kawakami, N
影响因子：
2.7
作者：
Grimes, AC;Stadt, HA;Kirby, ML
通讯作者：
Kirby, ML
缺乏周细胞会导致内皮增生和异常血管形态发生。
影响因子：
7.8
作者：
Hellstrom, M;Gerhardt, H;Kalen, M;Li, X;Eriksson, U;Wolburg, H;Betsholtz, C
通讯作者：
Betsholtz, C
影响因子：
20.1
作者：
Huang J;Davis EC;Chapman SL;Budatha M;Marmorstein LY;Word RA;Yanagisawa H
通讯作者：
Yanagisawa H
影响因子：
1.5
作者：
Armstrong, John J.;Larina, Irina V.;Dickinson, Mary E.;Zimmer, Warren E.;Hirschi, Karen K.
通讯作者：
Hirschi, Karen K.