A ticket to ride - Allele delivery by rail in secondary ruderal colonization by Arabidopsis arenosa
A ticket to ride - Allele delivery by rail in secondary ruderal colonization by Arabidopsis arenosa
复制标题
一张乘车票——拟南芥次生荒漠定植中的等位基因通过铁路传递
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
K. Bomblies
中科院分区：
文献类型：
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作者：
P. Baduel;B. Hunter;Sarang Yeola;K. Bomblies
人类创造的原始栖息地丰富，但对植物来说却充满挑战。然而，一些野生生境提供了网络走廊（如路边和铁路），可以促进成功适应的变种的快速远距离传播。在这里，我们使用转录组学和基因组学分析，结合遗传作图和转基因来了解拟南芥对铁路的适应。我们发现，通常多年生的羊草在铁路殖民后至少两次转变为快速循环，这是野生植物的一种常见适应。我们进一步表明，来自广泛分布的铁路殖民者的大量基因流可能有助于非野蛮类型的二次殖民化，突出了连通性如何影响适应性。我们发现繁殖抑制因子开花位点C （FLC）的表达缺失可能是广泛分布的铁路变异中快速循环的主要原因。然而，阿尔卑斯山的第二个铁路殖民者也循环迅速，但保持高FLC。这个群体中的一些等位基因编码无功能蛋白质，这表明FLC已经开始衰退，但大多数是功能性的。相反，该群体可能通过CONSTANS （CO）的衍生等位基因来规避FLC，这在该群体中显示了强有力的选择证据。重要的是，我们发现这个CO等位基因是通过基因流从广泛分布的野生动物中到达的，在那里它也曾经历过选择。这表明总体适应可能是渐进的，可能在两种情况下都是如此，首先出现FLC规避，随后出现FLC损失，但最终掩盖了其早期规避。这些铁路适应的快照突出表明，来自广泛分布的野生动物的基因流可以为当地基因型的后续适应提供机会。
Human-generated ruderal habitats are abundant, but challenging for plants. Some ruderal habitats, however, provide networked corridors (e.g. roadsides and railways) that can facilitate rapid long-distance spread of successfully adapted variants. Here we use transcriptomic and genomic analyses, coupled with genetic mapping and transgenics to understand adaptation to railways in Arabidopsis arenosa. We show normally perennial A. arenosa switched to rapid cycling, a common adaptation for ruderal plants, at least twice upon railway colonization. We further show substantial gene flow from a widely distributed railway colonist likely contributed to secondary colonization by a non-ruderal type, highlighting how connectivity can affect adaptability. We find loss of expression of the reproductive repressor FLOWERING LOCUS C (FLC) is likely primarily responsible for rapid cycling in the widely distributed railway variant. However, a second railway colonist in the Alps also cycles rapidly, but retains high FLC. Some alleles in this population encode non-functional proteins, suggesting FLC has started to decay, but most are functional. Instead, this population likely circumvents FLC via a derived allele of CONSTANS (CO), which shows strong evidence of selection in this population. Importantly, we find this CO allele arrived via gene flow from the widespread ruderal, where it was also previously under selection. This suggests ruderal adaptation may have been progressive, perhaps in both cases, with FLC-circumvention arising first, and FLC loss arising later but ultimately obscuring its earlier circumvention. These snapshots of railway adaptation highlight that gene flow from widespread ruderals can provide opportunities for subsequent adaptation by local genotypes.
影响因子：
30.8
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通讯作者：
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