Comprehensive analysis of LASS6 expression and prognostic value in ovarian cancer.
Comprehensive analysis of LASS6 expression and prognostic value in ovarian cancer.
复制标题
DOI：
10.1186/s13048-021-00868-z
复制
发表时间：
2021-09-07
影响因子：
4
通讯作者：
Yi J
中科院分区：
文献类型：
--
作者：
Xing J;Yi J
神经酰胺在肿瘤的发生、发展中起着重要作用。神经酰胺的合成需要LASS的参与。目前研究表明，不同的LASS家族成员在肿瘤中发挥不同的功能，特别是LASS 6，已被证实在乳腺癌、胃癌、黑色素瘤等中发挥关键作用，但对卵巢癌的研究非常有限。利用Oncomine、UALCAN、Kaplan-Meier Plotter和TIMER等生物信息学网络资源分析LASS 6的表达谱、预后价值和免疫浸润。应用Regulome Explorer和LinkedOmics数据库挖掘卵巢癌LASS 6相关基因，大卫软件进行聚类分析。利用STRING数据库构建了包含LASS 6的PPI网络。应用GEPIA数据库分析10个基因与LASS 6的相关性，并采用Kaplan-Meier逐步回归法分析其在卵巢癌预后中的价值。LASS 6在卵巢癌组织中表达上调，与卵巢癌的进展及预后不良有关。通过GO/KEGG聚类分析，我们还发现LASS 6可能影响钙离子通道及其转运途径。LASS 6参与的调控网络分析显示，7个关键基因的高表达与卵巢癌患者OS的不良预后相关，其中DEGS 1表达最显著。LASS 6可能在钙通道的调控中发挥重要作用，成为卵巢癌新的治疗靶点和潜在的预后指标。在线版本包含补充材料，可通过10.1186/s13048-021-00868-z获得。
Ceramide plays an important role in the occurrence and development of tumor. The synthesis of ceramide needs the participation of LASS. Current studies have shown that different LASS family members play different functions in tumors, especially LASS6, has been proved to play a key role in breast cancer, gastric cancer, melanoma and so on, but the research on ovarian cancer is very limited. Bioinformatics web resources, including Oncomine, UALCAN, Kaplan–Meier Plotter and TIMER were used to analyze the expression profile, prognostic value and immune infiltration of LASS6. The related genes of LASS6 in ovarian cancer were mined by Regulome Explorer and LinkedOmics database, and cluster analysis was done by DAVID. The PPI network involving LASS6 was constructed by STRING database. Finally, the correlation between 10 genes and LASS6 was analyzed by GEPIA database, and their prognostic value in ovarian cancer was analyzed by Kaplan–Meier plotter. The expression of LASS6 was up-regulated in ovarian cancer, which was related to the progression and poor prognosis of ovarian cancer. Through GO/KEGG cluster analysis, we also found that LASS6 may affect calcium ion channel and its transport pathways. The analysis of regulatory network involved in LASS6 showed that the high mRNAs of 7 key genes were associated with poor prognosis of OS in patients with ovarian cancer, among which DEGS1 was the most significant. LASS6 may play an important role in the regulation of calcium pathway and become a new therapeutic target and potential prognostic marker in ovarian cancer. The online version contains supplementary material available at 10.1186/s13048-021-00868-z.
登录
查看更多内容
DOI：
10.1038/nrm.2017.107
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Hannun YA;Obeid LM
通讯作者：
Obeid LM
影响因子：
4.6
作者：
Levy, Michal;Futerman, Anthony H.
通讯作者：
Futerman, Anthony H.
影响因子：
4.1
作者：
Mizutani, Y;Kihara, A;Igarashi, Y
通讯作者：
Igarashi, Y
影响因子：
4.4
作者：
Dybkaer K;Iqbal J;Zhou G;Geng H;Xiao L;Schmitz A;d'Amore F;Chan WC
通讯作者：
Chan WC
影响因子：
4.8
作者：
Fekry, Baharan;Jeffries, Kristen A.;Krupenko, Natalia I.
通讯作者：
Krupenko, Natalia I.