Coupled analysis of in vitro and histology tissue samples to quantify structure-function relationship.
Coupled analysis of in vitro and histology tissue samples to quantify structure-function relationship.
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DOI：
10.1371/journal.pone.0032227
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yener B
中科院分区：
文献类型：
--
作者：
Acar E;Plopper GE;Yener B
结构/功能关系是我们理解生物系统各个层面的基础，并驱动大多数（如果不是全部）检测，诊断和治疗疾病的技术。然而，在生物复杂性的组织水平上，我们遇到了结构/功能关系中的一个缺口：在细胞和亚细胞水平上积累了大量关于生物组织的详细信息，我们无法以一种解释这些结构执行的相应复杂生物功能的方式来组装它。为了帮助缩小这一信息差距，我们在这里定义了几个定量的时空特征，将组织结构与其相应的生物功能联系起来。人体组织样本的组织学图像和人体细胞三维培养物的荧光图像都用于比较体外培养模型与其相应人体组织的准确性。据我们所知，之前没有对组织学和体外样本进行定量比较的工作。根据组织结构的图论表示计算特征，并使用矩阵和张量因子分解方法以矩阵和高阶张量的形式分析数据，目标是区分脑，乳腺和骨组织的癌症和健康状态。我们还表明，我们的技术可以区分天然组织的结构组织和相应的体外工程细胞培养模型。
The structure/function relationship is fundamental to our understanding of biological systems at all levels, and drives most, if not all, techniques for detecting, diagnosing, and treating disease. However, at the tissue level of biological complexity we encounter a gap in the structure/function relationship: having accumulated an extraordinary amount of detailed information about biological tissues at the cellular and subcellular level, we cannot assemble it in a way that explains the correspondingly complex biological functions these structures perform. To help close this information gap we define here several quantitative temperospatial features that link tissue structure to its corresponding biological function. Both histological images of human tissue samples and fluorescence images of three-dimensional cultures of human cells are used to compare the accuracy of in vitro culture models with their corresponding human tissues. To the best of our knowledge, there is no prior work on a quantitative comparison of histology and in vitro samples. Features are calculated from graph theoretical representations of tissue structures and the data are analyzed in the form of matrices and higher-order tensors using matrix and tensor factorization methods, with a goal of differentiating between cancerous and healthy states of brain, breast, and bone tissues. We also show that our techniques can differentiate between the structural organization of native tissues and their corresponding in vitro engineered cell culture models.
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影响因子：
5.8
作者：
Demir, C;Gultekin, SH;Yener, B
通讯作者：
Yener, B
影响因子：
--
作者：
Lund AW;Bilgin CC;Hasan MA;McKeen LM;Stegemann JP;Yener B;Zaki MJ;Plopper GE
通讯作者：
Plopper GE
影响因子：
4.8
作者：
Bilgin, Cemal Cagatay;Bullough, Peter;Plopper, George E.;Yener, Buelent
通讯作者：
Yener, Buelent
DOI：
10.1109/4233.992163
发表时间：
2002-03-01
影响因子：
--
作者：
Esgiar, AN;Naguib, RNG;Murray, A
通讯作者：
Murray, A
影响因子：
5.8
作者：
Gunduz, Cigdem;Yener, Buelent;Gultekin, S. Humayun
通讯作者：
Gultekin, S. Humayun