The reaction center complex from the green sulfur bacterium Chlorobium tepidum: a structural analysis by scanning transmission electron microscopy.
The reaction center complex from the green sulfur bacterium Chlorobium tepidum: a structural analysis by scanning transmission electron microscopy.
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来自绿色硫细菌温热绿菌的反应中心复合物：通过扫描透射电子显微镜进行结构分析。
DOI：
10.1006/jmbi.1999.2925
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发表时间：
1999
影响因子：
5.6
通讯作者：
G. Tsiotis
中科院分区：
文献类型：
--
作者：
H. Rémigy;H. Stahlberg;D. Fotiadis;S. Müller;B. Wolpensinger;A. Engel;G. Hauska;G. Tsiotis
用角重构法对从绿硫菌中分离得到的反应中心（RC）配合物的三维结构进行了测定。纯化后的复合物含有PscA、PscC、PscB、PscD亚基和FMO蛋白。通过扫描透射电子显微镜（STEM）发现其质量为454 kDa，表明存在两个PscA亚基拷贝，一个PscB和PscD亚基拷贝，三个FMO蛋白和至少一个PscC亚基拷贝。在183 kDa处的附加质量峰表明FMO三聚体与RC配合物发生了共聚合。用STEM记录阴性染色的RC复合物的图像，并用多元统计分析进行比对和分类。主要类别的平均值表明，不同形态的细长颗粒（长=19 nm，宽=8 nm）是由绕长轴旋转引起的。从这些投影重建的3D地图可以可视化与一个FMO三聚体相关的RC复合物。第二个FMO三聚体可以相应地容纳以产生对称配合物，这种结构在少量侧面视图中观察到，并且被认为是RC配合物的完整形式。
The three-dimensional (3D) structure of the reaction center (RC) complex isolated from the green sulfur bacterium Chlorobium tepidum was determined from projections of negatively stained preparations by angular reconstitution. The purified complex contained the PscA, PscC, PscB, PscD subunits and the Fenna-Matthews-Olson (FMO) protein. Its mass was found to be 454 kDa by scanning transmission electron microscopy (STEM), indicating the presence of two copies of the PscA subunit, one copy of the PscB and PscD subunits, three FMO proteins and at least one copy of the PscC subunit. An additional mass peak at 183 kDa suggested that FMO trimers copurify with the RC complexes. Images of negatively stained RC complexes were recorded by STEM and aligned and classified by multivariate statistical analysis. Averages of the major classes indicated that different morphologies of the elongated particles (length=19 nm, width=8 nm) resulted from a rotation around the long axis. The 3D map reconstructed from these projections allowed visualization of the RC complex associated with one FMO trimer. A second FMO trimer could be correspondingly accommodated to yield a symmetric complex, a structure observed in a small number of side views and proposed to be the intact form of the RC complex.
DOI：
10.1002/jemt.1060090405
发表时间：
1988-08-01
期刊：
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE
影响因子：
--
作者：
RADERMACHER, M
通讯作者：
RADERMACHER, M
影响因子：
2.2
作者：
PENCZEK, P;RADERMACHER, M;FRANK, J
通讯作者：
FRANK, J